Evaluation of Antioxidant, Anti-Inflammatory and Cytoprotective Properties of Ethanolic Mint Extracts from Algeria on 7-Ketocholesterol-Treated Murine RAW 264.7 Macrophages
Evaluation of Antioxidant, Anti-Inflammatory and Cytoprotective Properties of Ethanolic Mint Extracts from Algeria on 7-Ketocholesterol-Treated Murine RAW 264.7 Macrophages
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DOI：
10.3390/antiox7120184
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发表时间：
2018-12-01
期刊：
影响因子：
7
通讯作者：
Lizard, Gerard
中科院分区：
文献类型：
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作者：
Brahmi, Fatiha;Nury, Thomas;Lizard, Gerard
本研究包括采用生物化学方法评估三种薄荷品种（Mentha spicata L. (MS)、Mentha pulegium L. (MP) 和 Mentha rotundifolia (L.) Huds (MR)）乙醇提取物对小鼠 RAW 264.7 巨噬细胞（一种从小鼠体内分离的转化巨噬细胞系）的抗氧化、抗炎和细胞保护特性。 被 Abelson 白血病病毒感染的 BALB/c 小鼠的腹水）。采用分光光度法测定总酚、黄酮和类胡萝卜素的含量。使用Radicaux Libres Kit (KRL (TM))、铁还原抗氧化能力(FRAP) 和2,2-二苯基-1-三硝基苯肼(DPPH) 测定法对抗氧化活性进行定量。 MS提取物显示出最高的总酚含量和最高的抗氧化能力，而MR提取物显示出最低的总酚含量和最低的抗氧化能力。在用不同浓度（25、50、100、200和400μg/mL）的乙醇薄荷提取物联合或不联合7-酮胆固醇（7KC；20μg/mL：50μM）处理24小时和48小时的小鼠RAW 264.7巨噬细胞上，对提取物的细胞保护和抗炎活性进行定量。在 7KC 处理下，结晶紫测试显示出对细胞生长的重要抑制作用。使用不同的乙醇薄荷提取物，这种副作用以剂量依赖性方式被强烈减弱，主要是在 48 小时时。 MS 提取物观察到最重要的细胞保护作用。此外，在24小时内测定脂多糖（LPS，0.2μg/mL）-、7KC（20μg/mL）上的乙醇薄荷提取物对细胞因子分泌（白细胞介素（IL）-6、IL-10、单核细胞趋化蛋白（MCP）-1、干扰素（IFN）-Y、肿瘤坏死因子（TNF）-α）的影响。 g/mL) 和 (7KC + LPS) 处理的 RAW 264.7 细胞。观察到薄荷提取物对细胞因子分泌的复杂影响。然而，与 LPS 处理的细胞相比，所有提取物都强烈减少 IL-6 分泌，其中两种提取物（MP 和 MR）还减少 MCP-1 和 TNF-α 分泌。然而，在 7KC 和 (7KC + LPS) 处理的细胞中没有观察到抗炎作用。总而言之，这些数据为阿尔及利亚薄荷对人类健康的潜在益处（尤其是抗氧化和细胞保护特性）提供了新的证据。
The present study consisted in evaluating the antioxidant, anti-inflammatory and cytoprotective properties of ethanolic extracts from three mint species (Mentha spicata L. (MS), Mentha pulegium L. (MP) and Mentha rotundifolia (L.) Huds (MR)) with biochemical methods on murine RAW 264.7 macrophages (a transformed macrophage cell line isolated from ascites of BALB/c mice infected by the Abelson leukemia virus). The total phenolic, flavonoid and carotenoid contents were determined with spectrophotometric methods. The antioxidant activities were quantified with the Kit Radicaux Libres (KRL (TM)), the ferric reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The MS extract showed the highest total phenolic content, and the highest antioxidant capacity, while the MR extract showed the lowest total phenolic content and the lowest antioxidant capacity. The cytoprotective and anti-inflammatory activities of the extracts were quantified on murine RAW 264.7 macrophages treated with 7-ketocholesterol (7KC; 20 mu g/mL: 50 mu M) associated or not for 24 h and 48 h with ethanolic mint extracts used at different concentrations (25, 50, 100, 200 and 400 mu g/mL). Under treatment with 7KC, an important inhibition of cell growth was revealed with the crystal violet test. This side effect was strongly attenuated in a dose dependent manner with the different ethanolic mint extracts, mainly at 48 h. The most important cytoprotective effect was observed with the MS extract. In addition, the effects of ethanolic mint extracts on cytokine secretion (Interleukin (IL)-6, IL-10, Monocyte Chemoattractant Protein (MCP)-1, Interferon (IFN)-Y, Tumor necrosis factor (TNF)-alpha) were determined at 24 h on lipopolysaccharide (LPS, 0.2 mu g/mL)-, 7KC (20 mu g/mL)- and (7KC + LPS)-treated RAW 264.7 cells. Complex effects of mint extracts were observed on cytokine secretion. However, comparatively to LPS-treated cells, all the extracts strongly reduce IL-6 secretion and two of them (MP and MR) also decrease MCP-1 and TNF-alpha secretion. However, no anti-inflammatory effects were observed on 7KC- and (7KC + LPS)-treated cells. Altogether, these data bring new evidences on the potential benefits (especially antioxidant and cytoprotective properties) of Algerian mint on human health.