Kruppel-Like Factor 2 Regulates Dendritic Cell Activation in Patients with Acute Coronary Syndrome
Kruppel-Like Factor 2 Regulates Dendritic Cell Activation in Patients with Acute Coronary Syndrome
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Kruppel 样因子 2 调节急性冠状动脉综合征患者的树突状细胞活化
DOI：
10.1159/000354496
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发表时间：
2013-01-01
影响因子：
--
通讯作者：
Li, Dazhu
中科院分区：
文献类型：
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作者：
Fang, Hongcheng;Lin, Jing;Li, Dazhu
目的：树突状细胞（Dendritic cells，DCs）的活化在动脉粥样硬化和冠心病中起重要作用，但其活化的调控机制尚不清楚。本研究的目的是评估转录因子Kruppel样因子2（KLF 2）在急性冠状动脉综合征中DC的促炎激活中的作用。方法和结果：本研究检测了正常人、稳定型心绞痛（SA）和急性冠脉综合征（ACS）患者外周血DC中CD 80和KLF 2的表达。我们的研究发现，与正常对照和SA相比，ACS患者DC中KLF 2的表达降低。ACS患者外周血CD 80表达明显增强。体外实验发现ox-LDL可增加CD 80表达，降低KLF 2表达。下调KLF 2表达可通过NF-κB途径增加CD 80表达。结论：这些观察结果表明KLF 2作为一种新的DC功能的负调节因子，它可能在ACS中DC激活中发挥重要作用。
Objective: Dendritic cells (DCs) activation is important in atherosclerosis and coronary heart disease, but the mechanisms regulating activation of dendritic cells remain largely unclear. The aim of this study was to evaluate the effect of transcription factor Kruppel-like factor 2 (KLF2) in the proinflammatory activation of DCs in acute coronary syndrome. Methods and Results: In this study, the expression of CD80 and KLF2 was detected in DCs in normal health controls, patients with stable angina (SA), and acute coronary syndrome (ACS). Our study found that compared with normal control and SA, KLF2 expression in DCs is reduced in patients with ACS. Moreover, the surface expression of CD80 was increased in ACS. In vitro experiment, we found that ox-LDL could increase CD80 expression and decrease KLF2 expression. Furthermore, down-regulated KLF2 could in turn increase CD80 expression via NF-κB pathway. Conclusions: These observations identify KLF2 as a novel negative regulator of DC function and it may play an essential role in DC activation in ACS.