Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea.
Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea.
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DOI：
10.1155/2012/731293
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Kubo T
中科院分区：
文献类型：
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作者：
Kimura T;Ono S;Kubo T
狼蛛的毒腺能产生多种生物活性肽。在这里，我们提出了鉴定毒液成分的测序克隆分离的cDNA文库制备的毒腺的智利常见的狼蛛，Grammostola rosea。在使用几个标准选择后，分析了4000个克隆中约1500个克隆的cDNA序列。从核苷酸序列预测了48个新的毒素样肽（GTx 1至GTx 7，以及GTx-TCTP和GTx-CRISP）。在这些肽中，24个毒素是ICK基序肽，11个肽是MIT 1样肽，7个是ESTX样肽。还获得了类似于JZTX-64、适体毒素、CRISP或TCTP的肽。GTx 3系列具有在脊椎动物MIT 1、Bv 8、prokineticins和无脊椎动物astakines中保守的半胱氨酸框架。GTx-CRISP是第一个从节肢动物毒液中鉴定出的CRISP样蛋白。实时荧光定量PCR结果显示，TCTP样肽的转录本在足前肌和毒腺中均有表达。此外，获得了一个独特的肽GTx 7 -1，其信号和前体序列基本上与HaTx 1相同。
Tarantula venom glands produce a large variety of bioactive peptides. Here we present the identification of venom components obtained by sequencing clones isolated from a cDNA library prepared from the venom glands of the Chilean common tarantula, Grammostola rosea. The cDNA sequences of about 1500 clones out of 4000 clones were analyzed after selection using several criteria. Forty-eight novel toxin-like peptides (GTx1 to GTx7, and GTx-TCTP and GTx-CRISP) were predicted from the nucleotide sequences. Among these peptides, twenty-four toxins are ICK motif peptides, eleven peptides are MIT1-like peptides, and seven are ESTX-like peptides. Peptides similar to JZTX-64, aptotoxin, CRISP, or TCTP are also obtained. GTx3 series possess a cysteine framework that is conserved among vertebrate MIT1, Bv8, prokineticins, and invertebrate astakines. GTx-CRISP is the first CRISP-like protein identified from the arthropod venom. Real-time PCR revealed that the transcripts for TCTP-like peptide are expressed in both the pereopodal muscle and the venom gland. Furthermore, a unique peptide GTx7-1, whose signal and prepro sequences are essentially identical to those of HaTx1, was obtained.