In vivo bioelectronic nose using transgenic mice for specific odor detection
In vivo bioelectronic nose using transgenic mice for specific odor detection
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使用转基因小鼠体内生物电子鼻进行特定气味检测
DOI：
10.1016/j.bios.2017.08.055
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发表时间：
2018-04-15
影响因子：
12.6
通讯作者：
Wang, Ping
中科院分区：
文献类型：
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作者：
Gao, Keqiang;Li, Songmin;Wang, Ping
嗅觉系统是一种自然的生物传感器，因为它的外周嗅觉神经元(OSN)对外界刺激做出反应，并将信号传输到嗅球(OB)，在那里它们被整合和处理。来自表达约1000种不同气味受体的OSN的轴突连接被精确地组织和分类到OB的1800个肾小球上，嗅觉信息从那里传递到中枢神经系统并被中枢神经系统感知。该方法具有特别高的灵敏度、特异度和快速性，可用于爆炸物检测。仿生嗅觉生物传感器利用嗅觉系统中的各种生物成分作为传感元件，具有很大的商业前景。在本研究中，我们利用带有绿色荧光蛋白(GFP)的基因标记的小鼠M72嗅觉感觉神经元作为传感组件，通过将微电极阵列(MEA)植入行为小鼠的OB，从M72嗅觉神经元获得长期的在体电生理记录。电生理反应显示出对气味检测的高可靠性、重复性和特异性，特别是对含有苯环的气味检测的高灵敏度。此外，我们的结果表明，它可以检测到低至10(-5)M的液体中的三硝基甲苯(TNT)，并能将TNT与其他结构相似的化学物质区分开来。因此，我们的研究表明，体内仿生嗅觉系统可以为提高嗅觉生物传感器的特异性和延长其工作寿命提供新的途径。
The olfactory system is a natural biosensor since its peripheral olfactory sensory neurons (OSNs) respond to the external stimuli and transmit the signals to the olfactory bulb (OB) where they are integrated and processed. The axonal connections from the OSNs expressing about 1000 different types of odorant receptors are precisely organized and sorted out onto 1800 glomeruli in the OB, from which the olfactory information is delivered to and perceived by the central nervous system. This process is carried out with particularly high sensitivity, specificity and rapidity, which can be used for explosive detection. Biomimetic olfactory biosensors use various biological components from the olfactory system as sensing elements, possessing great commercial prospects. In this study, we utilized the genetically labeled murine M72 olfactory sensory neurons with the green fluorescent protein (GFP) as sensing components and obtained long-term in vivo electro-physiological recordings from the M72 OSNs by implanting the microelectrode arrays (MEAs) into the behaving mouse's OB. The electro-physiological responses showed high reliability, reproducibility and specificity for odor detection, and particularly, the high sensitivity for the detection of odorants that contain benzene rings. Furthermore, our results indicated that it can detect trinitrotoluene (TNT) in liquid at a concentration as low as 10(-5) M and can distinguish TNT from other chemicals with a similar structure. Thus our study demonstrated that the in vivo biomimetic olfactory system could provide novel approaches to enhancing the specificity and increasing working lifespan of olfactory biosensors capable of detecting explosives.