Design criteria for studies examining individual fatty acid effects on cardiovascular disease risk factors: Human and animal studies
Design criteria for studies examining individual fatty acid effects on cardiovascular disease risk factors: Human and animal studies
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DOI：
10.1093/ajcn/65.5.1590s
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发表时间：
1997-01-01
影响因子：
7.1
通讯作者：
Dietschy, John
中科院分区：
文献类型：
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作者：
Kris-Etherton, Penny M.;Dietschy, John
旨在检查人类和动物个体脂肪酸效应的研究严重依赖于受试者或动物种属、实验饮食和所用的实验设计。对于人类和动物研究，受试者和动物的数量必须足以达到统计学显著性，并且受试者和动物必须适当分组（例如，年龄、性别和胆固醇反应性）。在动物研究中，必须选择适当的种属，因为有些种属是不可接受的模型。在人类和动物研究中，必须非常注意实验饮食的设计。试验饲料必须严格控制，必须符合营养规格，并通过化学分析进行验证。理想情况下，只有感兴趣的脂肪酸应该在测试饮食中变化。两个实验设计是适当的：交叉和平行臂设计。喂食期必须是终点稳定的足够持续时间。注意这些研究设计问题是必要的，以达到有意义的结论，个别脂肪酸的影响。
Studies designed to examine individual fatty acid effects in humans and animals are critically dependent on subjects or animal species, experimental diets, and the experimental design used. For both human and animal studies, the numbers of subjects and animals must be adequate for achieving statistical significance and the subjects and animals must be grouped appropriately (eg, age, sex, and cholesterol responsiveness). In animal studies the appropriate species must be selected because some species are unacceptable models. In both human and animal studies, great attention must be paid to the design of the experimental diets. Test diets must be tightly controlled and nutrient specifications must be met and verified by chemical analysis. Ideally, only the fatty acid of interest should vary among the test diets. Two experimental designs are appropriate: crossover and parallel-arm designs. Feeding periods must be adequate duration of stabilization of endpoints. Attention to these study design issues is imperative for meaningful conclusions to be reached about the effects of individual fatty acids.