On-chip processing of droplets for surface plasmon resonance analysis
On-chip processing of droplets for surface plasmon resonance analysis
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DOI：
10.1016/j.snb.2013.09.027
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发表时间：
2014
影响因子：
8.4
通讯作者：
K. Okubo;Yoshitaka Yoshizumi;K. Asakawa;H. Suzuki;M. Yokokawa
中科院分区：
文献类型：
--
作者：
K. Okubo;Yoshitaka Yoshizumi;K. Asakawa;H. Suzuki;M. Yokokawa
处理液滴形式的溶液，并基于表面等离子体共振（SPR）进行分析物分子传感。使用具有不同微流体功能的两种类型的装置。进行诸如测量溶液体积、混合溶液和顺序分析的程序，然后进行SPR感测。通过在芯片上稀释100和1000 mM葡萄糖溶液制备不同浓度的溶液，观察到反射率变化的明显差异。在反射率的变化与葡萄糖浓度之间观察到线性关系。该装置的功能性也通过检测用量子点（Q-dot）（QD-DNA复合物）标记的DNA来测试，所述量子点（Q-dot）（QD-DNA复合物）伴随DNA与固定在感测区的金表面上的硫醇封端的单链DNA的杂交。在单个流动通道中进行包括含有葡萄糖和QD-DNA复合物的塞的顺序分析。当水栓被葡萄糖栓取代时，反射率迅速变化，而QD-DNA复合物的结合显示出反射率的逐渐增加。
Solutions in the form of liquid droplets were processed and analyte molecule sensing was performed, based on surface plasmon resonance (SPR). Two types of devices with different microfluidic functions were used. Procedures such as measurement of solution volume, mixing of solutions, and sequential analyses were conducted, followed by SPR sensing. A distinct difference in the variation of reflectivity was observed with solutions of different concentrations prepared by diluting 100 and 1000 mM glucose solutions on the chip. A linear relationship was observed between the change in reflectivity and glucose concentration. The functionality of this device was also tested by detecting the DNA that was tagged with a quantum dot (Q-dot) (QD-DNA complex) accompanying the DNA hybridization with thiol-terminated single-stranded DNA immobilized on the gold surface of the sensing region. Sequential analysis of plugs including those containing glucose and the QD-DNA complex in a single flow channel was carried out. Reflectivity changed rapidly when a water plug was replaced with a glucose plug, whereas the binding of the QD-DNA complex showed a gradual increase in reflectivity.