Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans.
Conjugal transfer of broad-host-range incompatibility group P and Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans.
复制标题
将广泛宿主范围的不相容性 P 组和 Q 组质粒从大肠杆菌共轭转移至放线放线菌伴生放线杆菌。
DOI：
10.1128/iai.61.8.3544-3547.1993
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发表时间：
1993
影响因子：
3.1
通讯作者：
Fine,DH
中科院分区：
文献类型：
--
作者：
Goncharoff,P;Yip,JK;Wang,H;Schreiner,HC;Pai,JA;Furgang,D;Stevens,RH;Figurski,DH;Fine,DH
提出了第一个将大肠杆菌 DNA 结合转移到牙周病原体放线放线杆菌的例子。不相容性组 P (IncP) 质粒 RK2 的衍生物成功从大肠杆菌供体转移至放线放线菌受体。所得放线菌伴生转接合子将质粒转移回大肠杆菌受体。 IncP 转移功能也用于反式将 IncQ 质粒 pBK1 从大肠杆菌转移到 A. actinomycetemcomitans。 IncP 和 IncQ 质粒均以高频率（每个供体 0.3 至 0.5 个转接合子）转移至 A. actinomycetemcomitans 中，并且未显示出总体删除、插入或重排。对 A. actinomycetemcomitans 转接合菌株的各种抗生素的 MIC 测定表明氨苄青霉素、氯霉素和卡那霉素抗性决定簇的表达。
The first example of conjugal transfer of DNA from Escherichia coli to the periodontal pathogen Actinobacillus actinomycetemcomitans is presented. Derivatives of the incompatibility group P (IncP) plasmid RK2 successfully transferred from an E. coli donor to an A. actinomycetemcomitans recipient. The resulting A. actinomycetemcomitans transconjugants transferred the plasmids back to E. coli recipients. The IncP transfer functions were also used in trans to mobilize the IncQ plasmid pBK1 from E. coli to A. actinomycetemcomitans. The IncP and IncQ plasmids both transferred into A. actinomycetemcomitans at high frequencies (0.3 to 0.5 transconjugants per donor) and showed no gross deletions, insertions, or rearrangements. Determinations of MICs of various antibiotics for the A. actinomycetemcomitans transconjugant strains demonstrated the expression of ampicillin, chloramphenicol, and kanamycin resistance determinants.