Sustained apoptosis in human cardiac allografts despite histologic resolution of rejection.
Sustained apoptosis in human cardiac allografts despite histologic resolution of rejection.
复制标题
尽管组织学上消除了排斥反应，但人心脏同种异体移植物仍持续凋亡。
DOI：
10.1097/01.tp.0000084824.70320.da
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Bond,Meredith
中科院分区：
文献类型：
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作者：
Masri,SofiaC;Yamani,MohamadH;Russell,MaryA;Ratliff,NormanB;Yang,Jiacheng;Almasan,Alex;Apperson-Hansen,Carolyn;Li,Jianbo;Starling,RandallC;McCarthy,Patrick;Young,JamesB;Bond,Meredith
背景：我们研究了心脏移植排斥反应期间和消退后细胞凋亡的发生。细胞凋亡可以发挥不同的作用，在移植物的生存取决于靶细胞，因此，我们也确定了细胞类型involved.Methods. endomyosis活检标本进行了评估，在第一个6个月后移植如下：组I，没有当前或先前的排斥反应，组II，在一次发作的中度排斥反应，组III，在一次发作的中度排斥反应。结果：第二组和第三组细胞凋亡活性显著增加，表现为caspase-8和caspase-3活性增加;而在I组中未检测到活化的caspase-3。仅在组II和组III中检测到活化的caspase-3。末端脱氧核苷酸转移酶介导的dUTP缺口末端标记检测组II和III，但不是组I，主要是在炎症cells. Conclusions. increased caspase活性和细胞凋亡的浸润细胞不仅发生在急性心脏同种异体移植排斥反应，但持续组织学决议后。因此，程序性细胞死亡发生在组织学消退期之外，并可能在排斥过程的调节中发挥作用。
Background.We investigated the occurrence of apoptosis during and after resolution of cardiac allograft rejection. Apoptosis could play different roles in graft survival depending on the target cells; thus, we also determined the cell types involved.Methods.Endomyocardial biopsy specimens were evaluated during the first 6 months after transplantation as follows: group I, no current or prior rejection; group II, during an episode of moderate rejection; and group III, histologic resolution after an episode of moderate rejection.Results.Groups II and III showed significantly increased apoptotic activity, indicated by increased caspase-8 and caspase-3 activity; however, activated caspase-3 was undetectable in group I. Activated caspase-3 was detected only in groups II and III. Terminal deoxynucleotide transferase-mediated dUTP nick-end labeling was detected in groups II and III but not group I and predominantly in inflammatory cells.Conclusions.Increased caspase activity and apoptosis of infiltrating cells not only occurs during acute cardiac allograft rejection but persists after histologic resolution. Thus, programmed cell death occurs beyond the period of histologic resolution and may play a role in regulation of the rejection process.