EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD
EID-2, a novel member of the EID family of p300-binding proteins inhibits transactivation by MyoD
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DOI：
10.1016/j.gene.2003.06.001
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发表时间：
2003-10-30
期刊：
影响因子：
3.5
通讯作者：
MacLellan, WR
中科院分区：
文献类型：
--
作者：
Ji, AM;Dao, D;MacLellan, WR
骨骼肌分化依赖于Rb和p300的表达。我们最近克隆了一种名为EID-1的新型肌肉分化抑制剂，它与这两种因子相互作用。在相关分子的数据库搜索中，我们克隆并鉴定了一个新的EID-1家族成员EID-2。该28-kDa蛋白质编码236个氨基酸的蛋白质，其C-末端与EID-1具有显著的相似性。EID-2在成人心脏和大脑中表现出高水平的发育调节表达。EID-2的过表达通过抑制MyoD依赖性抑制肌肉特异性基因表达。转录。这种对基因表达的抑制作用可以通过EID-2与p300的乙酰转移酶活性相关并抑制p300的乙酰转移酶活性来解释。这些数据表明，EID-1和EID-2代表了一个新的蛋白家族，通过p300依赖性机制负调节分化。(C)2003 Elsevier B. V.保留所有权利。
Skeletal muscle differentiation has been shown to be dependent on the expression of Rb and p300. We recently cloned a novel inhibitor of muscle differentiation called EID-1, which interacted with both of these factors. In a database search for related molecules, we have cloned and characterized a new EID-1 family member, EID-2. This 28-kDa protein encodes a 236-amino-acid protein with significant similarity to EID-1 in its C-terminus. EID-2 displays developmentally regulated expression with high-levels in adult heart and brain. Overexpression of EID-2 inhibits muscle-specific gene expression through inhibition of MyoD-dependent. transcription. This inhibitory effect on gene expression can be explained by EID-2's ability to associate with and inhibit the acetyltransferase activity of p300. These data suggest that EID-1 and -2 represent a novel family of proteins that negatively regulate differentiation through a p300-dependent mechanism. (C) 2003 Elsevier B.V. All rights reserved.