Localized translation near the mitochondrial outer membrane: An update.
Localized translation near the mitochondrial outer membrane: An update.
复制标题
DOI：
10.1080/15476286.2015.1058686
复制
发表时间：
2015
期刊：
影响因子：
4.1
通讯作者：
Arava Y
中科院分区：
文献类型：
--
作者：
Lesnik C;Golani-Armon A;Arava Y
蛋白质在其活性位点附近的局部合成已经在许多生物系统中得到证实，并且对细胞功能具有不同的贡献。近年来的研究表明，数百种与线粒体相关的蛋白质是由线粒体外膜附近的胞质核糖体合成的，这表明在该细胞位点也发生了定位翻译。此外，去年在酵母、果蝇和人类细胞中发现了参与这一过程的核心因子。在此，我们回顾了线粒体外膜胞质侧的本地化翻译的实验证据，此外，我们描述了参与这一过程的因素，并讨论了不同物种之间的保护机制。我们还描述了本地化的翻译和进口到线粒体之间的关系，并建议研究途径，超越共翻译进口。最后，我们讨论了未来的挑战，在本地化的翻译和其生理意义的机制。
Local synthesis of proteins near their activity site has been demonstrated in many biological systems, and has diverse contributions to cellular functions. Studies in recent years have revealed that hundreds of mitochondria-destined proteins are synthesized by cytosolic ribosomes near the mitochondrial outer membrane, indicating that localized translation also occurs at this cellular locus. Furthermore, in the last year central factors that are involved in this process were identified in yeast, Drosophila, and human cells. Herein we review the experimental evidence for localized translation on the cytosolic side of the mitochondrial outer membrane; in addition, we describe the factors that are involved in this process and discuss the conservation of this mechanism among various species. We also describe the relationship between localized translation and import into the mitochondria and suggest avenues of study that look beyond cotranslational import. Finally we discuss future challenges in characterizing the mechanisms for localized translation and its physiological significance.