Expression of basic fibroblast growth factor in normal human tissues.
Expression of basic fibroblast growth factor in normal human tissues.
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发表时间：
1990-12
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通讯作者：
C. C. Cardo-C.;Israel Vlodavsky;A. Friedman;D. Hicklin;Zvi Fuks
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作者：
C. C. Cardo-C.;Israel Vlodavsky;A. Friedman;D. Hicklin;Zvi Fuks
采用免疫组织化学方法研究正常人体组织新鲜冰冻切片中碱性成纤维细胞生长因子（bFGF）的分布。使用特异性抗 bFGF 小鼠单克隆抗体进行免疫检测，该抗体在蛋白质印迹分析中发现与重组人 bFGF 发生反应，并特异性中和 bFGF 对牛血管内皮细胞的有丝分裂活性。正常人体组织中bFGF的表达在各种大小血管的基底膜中普遍检测到，但在多种测试组织的表皮或上皮基底膜中未发现。血管中的定位强度和模式在各种组织中是一致的，但在血管床的不同区域之间有所不同。虽然在大中型血管中观察到均匀且强烈的免疫反应性，但在毛细血管中发现表达的异质性。在分支毛细血管中观察到最强烈的免疫反应性。内皮细胞染色是异质的并且在不同区域中存在差异。在心肌纤维、中型血管的平滑肌细胞、肠道和子宫肌层、中枢神经系统神经元和小脑浦肯野细胞以及支气管、结肠、子宫内膜和皮肤汗腺导管的上皮细胞中也发现了强的 bFGF 染色。 bFGF 在多种器官的细胞外区室中的存在可能在血管生成中发挥作用。然而，bFGF 在实质细胞中的功能仍有待确定。
The distribution of basic fibroblast growth factor (bFGF) was studied immunohistochemically in fresh frozen sections of normal human tissues. Immunodetection was performed with a specific anti-bFGF mouse monoclonal antibody that was found to react with recombinant human bFGF in Western blot analysis, and to specifically neutralize the mitogenic activity of bFGF on bovine vascular endothelial cells. Expression of bFGF on normal human tissues was ubiquitously detected in the basement membranes of all size blood vessels, but was not found in epidermal or epithelial basement membranes of a variety of tissues tested. Intensity and patterns of localization in blood vessels was consistent in various tissues, but varied among different regions of the vascular bed. Whereas homogeneous and intense immunoreactivity were observed in large and intermediate size blood vessels, heterogeneity of expression was found in capillaries. The most intense immunoreactivity was observed in branching capillaries. Endothelial cell staining was heterogeneous and varied in different regions. Strong staining for bFGF was also found in cardiac muscle fibers, smooth muscle cells of mid-size blood vessels, the gut and the myometrium, in central nervous system neurons and cerebellar Purkinje cells, and on epithelial cells of the bronchi, colon, endometrium, and sweat gland ducts of the skin. The presence of bFGF in the extracellular compartment of a diverse variety of organs may play a role in angiogenesis. However, the function of bFGF in parenchymal cells remains to be determined.