RESTRICTION OF EPSTEIN-BARR VIRUS-SPECIFIC CYTO-TOXIC T-CELLS BY HLA-A, HLA-B, AND HLA-C MOLECULES
RESTRICTION OF EPSTEIN-BARR VIRUS-SPECIFIC CYTO-TOXIC T-CELLS BY HLA-A, HLA-B, AND HLA-C MOLECULES
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DOI：
10.1016/0198-8859(89)90099-2
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发表时间：
1989-10-01
期刊：
影响因子：
2.7
通讯作者：
SONDEL, PM
中科院分区：
文献类型：
--
作者：
CHEN, BP;LAM, V;SONDEL, PM
使用 Epstein-Barr 病毒转化的 B 淋巴母细胞系 (EBV-LCL) 721 的 HLA 丢失变体作为靶细胞来鉴定 EBV-LCL 特异性细胞毒性 T 细胞利用的 HLA 分子。对有限稀释铺板的细胞毒性 T 细胞的分裂培养分析显示，带有 HLA-A2 或 -B5 分子的 HLA 丢失变体被杀死，受 HLA-B5 分子限制的细胞毒性 T 细胞的频率高出 10 倍。克隆分析证实了HLA-A2或-B5对一些细胞毒性T细胞克隆的限制，并鉴定了对不表达HLA-A或-B但表达HLA-C决定簇的靶细胞具有溶细胞作用的细胞毒性T细胞克隆。因此，我们的结果表明HLA-B5限制性决定簇对HLA缺失变体供体中EBV诱导的抗原具有免疫优势，并提供证据表明HLA-C分子也可以作为EBV-LCL特异性细胞毒性T细胞的限制性决定簇。
HLA-loss variants of an Epstein-Barr virus-transformed B-lymphoblastoid cell line (EBV-LCL) 721 were used as target cells to identify HLA molecules utilized by EBV-LCL-specific cytotoxic T cells. Split culture analysis of cytotoxic T cells plated at limiting dilution showed killing of HLA-loss variants bearing either HLA-A2 or -B5 molecules, with 10 times higher frequency of cytotoxic T cells restricted by the HLA-B5 molecule. Clonal analysis confirmed the restriction by HLA-A2 or -B5 of some cytotoxic T-cell clones and identified cytotoxic T-cell clones cytolytic for target cells which do not express HLA-A or -B but do express the HLA-C determinant. Thus, our results show immunodominance of the HLA-B5 restriction determinant for EBV-induced antigens in the donor of the HLA-loss variants and provide evidence that the HLA-Cmolecule can also serve as restriction determinant for EBV-LCL-specific cytotoxic T cells.