Examination of the leaf proteome during flooding stress and the induction of programmed cell death in maize.
Examination of the leaf proteome during flooding stress and the induction of programmed cell death in maize.
复制标题
洪水胁迫期间叶片蛋白质组的检查和玉米程序性细胞死亡的诱导
DOI：
10.1186/1477-5956-12-33
复制
发表时间：
2014
期刊：
影响因子：
2
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Chen Y;Chen X;Wang H;Bao Y;Zhang W
玉米是世界范围内的主要经济作物，洪水造成了大量的作物损失。在逆境中，细胞程序性死亡（PCD）是植物适应逆境的有效途径。为了鉴定玉米叶片中与洪水胁迫相关的PCD蛋白，采用二维荧光差凝胶电泳（2D-DIGE）和质谱技术进行了蛋白质组学分析。采用比较蛋白质组学与生理生化分析相结合的方法对玉米叶片进行了水患胁迫分析。Fv/Fm、qP、qN和相对含水量（RWC）在淹水胁迫下发生改变，体内H2O2含量增加。此外，DNA阶梯检测表明，在驱油处理下发生了PCD。通过2D-DIGE凝胶分析玉米叶片蛋白质组，共分离到32个差异表达点，通过MALDI-TOF/TOF MS成功鉴定出31个差异表达点，代表28个蛋白。所鉴定的蛋白质与能量代谢和光合作用、PCD、植物激素和多胺有关。为了更好地表征翻译控制肿瘤蛋白（TCTP）在胁迫反应中PCD中的作用，我们通过胁迫诱导的PCD检测了不同植物的mRNA表达。热胁迫诱导的水稻原生质体、烟草花叶病毒侵染的烟草叶片、黑暗诱导的水稻和拟南芥叶片均表现出PCD活性，TCTP表达量均有不同程度的增加。淹水后，玉米叶片中s -腺苷甲硫氨酸合成酶2 （SAMS2）和s -腺苷甲硫氨酸脱羧酶（SAMDC） mRNA表达量增加，而ACC合成酶（ACS）和ACC氧化酶（ACO） mRNA表达量未见增加。最后，玉米叶片中乙烯和多胺浓度随淹水处理而升高。在洪水胁迫下，光合系统受到破坏，导致能量代谢中断，明显的光合作用下降也可能归因于ROS的产生。所观察到的PCD可能受到TCTP的调控，H2O2可能在洪水胁迫下的TCTP诱导中起作用。此外，SAMS2表达的增加与淹水处理期间多胺合成的增加密切相关。
Maize is a major economic crop worldwide, with substantial crop loss attributed to flooding. During a stress response, programmed cell death (PCD) can be an effective way for plants better adapt. To identify flooding stress related PCD proteins in maize leaves, proteomic analysis was performed using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry. Comparative proteomics was combined with physiological and biochemical analysis of maize leaves under flooding stress. Fv/Fm, qP, qN and relative water content (RWC) were found to be altered in response to flooding stress, with an increase in H2O2 content noted in vivo. Furthermore, DNA ladder detection indicated that PCD had occurred under flooding treatment. The maize leaf proteome was analyzed via 2D-DIGE gel, with a total of 32 differentially expressed spots isolated, 31 spots were successfully identified via MALDI-TOF/TOF MS which represent 28 proteins. The identified proteins were related to energy metabolism and photosynthesis, PCD, phytohormones and polyamines. To better characterize the role of translationally controlled tumor protein (TCTP) in PCD during a stress response, mRNA expression was examined in different plants by stress-induced PCD. These included heat stress induced rice protoplasts, Tobacco Mosaic Virus infected tobacco leaves and dark induced rice and Arabidopsis thaliana leaves, all of which showed active PCD, and TCTP expression was increased in different degrees. Moreover, S-adenosylmethionine synthase 2 (SAMS2) and S-adenosylmethionine decarboxylase (SAMDC) mRNA expression were also increased, but ACC synthase (ACS) and ACC oxidase (ACO) mRNA expression were not found in maize leaves following flooding. Lastly, ethylene and polyamine concentrations were increased in response to flooding treatment in maize leaves. Following flooding stress, the photosynthetic systems were damaged, resulting in a disruption in energy metabolism, with the noted photosynthetic decline also possibly attributed to ROS production. The observed PCD could be regulated by TCTP with a possible role for H2O2 in TCTP induction under flooding stress. Additionally, increased SAMS2 expression was closely associated with an increased polyamine synthesis during flooding treatment.