Use of Spent Mushroom Substrate for Production of Bacillus thuringiensis by Solid-State Fermentation
Use of Spent Mushroom Substrate for Production of Bacillus thuringiensis by Solid-State Fermentation
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DOI：
10.1603/ec13276
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发表时间：
2014-02
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通讯作者：
Songqing Wu;Y. Lan;Dongmei Huang;Yan Peng;Zhipeng Huang;Lei Xu;I. Gelbič;R. Carballar-Lejarazú;X. Guan;Lingling Zhang;Shuangquan Zou
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作者：
Songqing Wu;Y. Lan;Dongmei Huang;Yan Peng;Zhipeng Huang;Lei Xu;I. Gelbič;R. Carballar-Lejarazú;X. Guan;Lingling Zhang;Shuangquan Zou
摘要本研究旨在探索一种经济高效的固态发酵大规模生产苏云金芽孢杆菌（Bt）的方法。以食用菌糠（SMS）为原料，设计了4种SMS培养基组合。在最佳培养基上对发酵条件进行了优化，确定了最佳发酵条件为：温度32°C，初始pH值6，水分含量50%，筛料比1：3，接种量0.5ml。大规模生产B。苏云金在优化的培养基和优化的条件下对Israelensis（Bti）LLP 29进行了转化。研究中观察到该产品的高毒性（1，487国际毒性单位/毫克）和长的杀幼虫持久性，这说明基于SMS的固态发酵培养基对于Bt基生物农药的大规模工业生产是高效和经济的。生产1公斤Bt的成本约为0.075美元。
ABSTRACT The aim of this study was to explore a cost-effective method for the mass production of Bacillus thuringiensis (Bt) by solid-state fermentation. As a locally available agroindustrial byproduct, spent mushroom substrate (SMS) was used as raw material for Bt cultivation, and four combinations of SMS-based media were designed. Fermentation conditions were optimized on the best medium and the optimal conditions were determined as follows: temperature 32°C, initial pH value 6, moisture content 50%, the ratio of sieved material to initial material 1:3, and inoculum volume 0.5 ml. Large scale production of B. thuringiensis subsp. israelensis (Bti) LLP29 was conducted on the optimal medium at optimal conditions. High toxicity (1,487 international toxic units/milligram) and long larvicidal persistence of the product were observed in the study, which illustrated that SMS-based solid-state fermentation medium was efficient and economical for large scale industrial production of Bt-based biopesticides. The cost of production of 1 kg of Bt was approximately US$0.075.