ZmDof30 Negatively Regulates the Promoter Activity of the Pollen-Specific Gene Zm908.
ZmDof30 Negatively Regulates the Promoter Activity of the Pollen-Specific Gene Zm908.
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ZmDof30 负调控花粉特异性基因 Zm908 的启动子活性
DOI：
10.3389/fpls.2017.00685
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发表时间：
2017
影响因子：
5.6
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Peng J;Qi X;Chen X;Li N;Yu J
玉米花粉优势基因Zm 908是一个新的小肽基因，在花粉萌发和花粉管生长过程中起重要作用。本研究旨在探讨Zm 908的调控机制。对Zm 908的启动子进行了克隆和分析。对一系列截短的启动子在转基因烟草不同组织中的活性分析表明，Zm 908启动子是花粉特异性的，并且-126至-68区域是花粉表达的关键区域。对-126 ~-68区域的5′端缺失分析表明，-126 ~-102区域是一个转录抑制元件。ZmDof 30主要在花粉和整个花药中表达，被克隆和表征。ZmDof 30-GFP定位于玉米原生质体的细胞核，在酵母系统中不具有转录激活活性。ZmDof 30在体内外均能与含有Zm 908启动子-126 ~+58区的p184序列中的AAAG元件结合，并负调控烟草叶片中p184的活性。总的来说，ZmDof 30在花粉中可能作为Zm 908的转录抑制因子发挥作用，这些结果可以提供对Zm 908基因调控的更好理解。此外，花粉特异性Zm 908启动子可能对基因工程雄性不育有价值。
The maize (Zea mays) pollen-predominant gene Zm908, a novel small-peptide gene, was reported to play critical roles in pollen germination and pollen tube growth in our previous work. In this study, we aimed to explore the regulatory mechanism of Zm908. The putative promoter of Zm908 was cloned and analyzed. The activity analysis of a series of promoter truncations in different tissues of transgenic tobacco plants indicated that the Zm908 promoter is pollen-specific and that the –126 to –68 region is crucial for pollen expression. The 5′ deletion analysis of the –126 to –68 region revealed that the –126 to –102 region functions as a transcriptional suppression element. ZmDof30, which is predominantly expressed in pollen and whole anthers, was cloned and characterized. ZmDof30-GFP localized to the nuclei of maize protoplasts and possessed no transcriptional activation activity in a yeast system. ZmDof30 could bind to the AAAG elements in p184 sequence containing the –126 to +58 region of the Zm908 promoter in vitro and in vivo, and negatively regulated p184 activity in tobacco leaves. Collectively, ZmDof30 may function as a Zm908 transcriptional repressor in pollen, and these results may provide a better understanding of the regulation of the Zm908 gene. Additionally, the pollen-specific Zm908 promoter may be valuable for genetically engineering male sterility.