Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre
Microbial community structure and diversity in deep-sea hydrothermal vent sediments along the Eastern Lau Spreading Centre
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DOI：
10.1007/s13131-013-0276-6
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发表时间：
2013-02
影响因子：
1.4
通讯作者：
M. Wei;Ru-bing Zhang;Yuguang Wang;Hou Ji;Jia Zheng;Xinhua Chen;Hong-bo Zhou
中科院分区：
文献类型：
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作者：
M. Wei;Ru-bing Zhang;Yuguang Wang;Hou Ji;Jia Zheng;Xinhua Chen;Hong-bo Zhou
本研究的目的是调查五个活跃的热液田沉积物中的微生物结构和微生物的分布，沿着东劳扩展中心（ELSC）在劳盆地（西南太平洋）。通过变性梯度凝胶电泳（DGGE）和16 S rRNA基因克隆文库分析对微生物群落进行了调查。DGGE图谱揭示了5个深海热液区沉积物样品中微生物群落结构的差异。DGGE图谱的聚类分析将五个热液样品分为两组。构建了四个不同的16 S rRNA基因克隆文库，代表两个选定的热液样品（19- 4 TVG 8和19- 4 TVG 11）。分别从166个和160个细菌克隆中鉴定出23个和32个细菌型，包括变形菌门、拟杆菌门、厚壁菌门、硝化螺旋菌门和浮游菌门。变形菌门在两个细菌文库中都占优势，γ-变形菌门占优势。分别从160个和130个古菌克隆中获得了31个和25个系统发育型，包括杂项泉古菌群、海洋I和III群、海洋底栖E群、陆地温泉泉古菌群和深海热液喷口广古菌群。这些结果表明，各种克隆参与硫循环，这表明硫化合物的循环和利用可能广泛发生在劳盆地深海热液生态系统。
The aim of this study is to investigate microbial structures and diversities in five active hydrothermal fields’ sediments along the Eastern Lau Spreading Centre (ELSC) in the Lau Basin (southwest Pacific). Microbial communities were surveyed by denatured gradient gel electrophoresis (DGGE) and clone library analysis of 16S rRNA genes. The differences in microbial community structures among sediment samples from the five deep-sea hydrothermal sites were revealed by DGGE profiles. Cluster analysis of DGGE profiles separated the five hydrothermal samples into two groups. Four different 16S rRNA gene clone libraries, representing two selected hydrothermal samples (19-4TVG8 and 19-4TVG11), were constructed. Twenty-three and 32 phylotypes were identified from166 and 160 bacterial clones respectively, includingProteobacteria, Bacteroidetes, Firmicutes, NitrospiraeandPlanctomycetes. The phylumProteobacteriais dominant in both bacterial libraries with a predominance of Gamma-Proteobacteria. A total of 31 and 25 phylotypes were obtained from 160 and 130 archaeal clones respectively, including Miscellaneous Crenarchaeotic Group, Marine Group I and III, Marine Benthic Group E, Terrestrial Hot Spring Crenarchaeota and Deep-sea Hydrothermal Vent Euryarchaeota. These results show a variety of clones related to those involved in sulfur cycling, suggesting that the cycling and utilization of sulfur compounds may extensively occur in the Lau Basin deep-sea hydrothermal ecosystem.