Proteomic analysis of pancreas derived from adult cloned pig
Proteomic analysis of pancreas derived from adult cloned pig
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DOI：
10.1016/j.bbrc.2007.11.114
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发表时间：
2008-02-08
影响因子：
3.1
通讯作者：
Lee, Kyung-Kwang
中科院分区：
文献类型：
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作者：
Chae, Jung-Il;Cho, Young Keun;Lee, Kyung-Kwang
动物克隆的潜在医学应用包括异种移植，但控制猪器官发育的复杂分子级联尚未完全了解。尽管如此，克隆猪的器官可能适合移植到人类身上已经变得很明显。在这项研究中，我们研究了成年克隆猪的胰腺体细胞核移植（SCNT）开发使用双向电泳（2-DE）和蛋白质印迹。蛋白质组学分析揭示了69个差异调节蛋白，包括这些凋亡相关的物种，如膜联蛋白，核纤层蛋白和热休克蛋白，这是一致上调的SCNT样品。在SCNT胰腺中下调的蛋白质包括过氧化物酶和过氧化氢酶。Western blot结果表明，几种抗氧化酶和抗凋亡蛋白在SCNT胰腺下调，而几个半胱天冬酶上调。总之，这些数据表明，活性氧（ROS）在成年克隆猪胰腺中的积累导致细胞凋亡。(C)2007爱思唯尔公司All rights reserved.
The potential medical applications of animal cloning include xenotransplantation, but the complex molecular cascades that control porcine organ development are not fully understood. Still, it has become apparent that organs derived from cloned pigs may be suitable for transplantation into humans. In this study, we examined the pancreas of an adult cloned pig developed through somatic cell nuclear transfer (SCNT) using two-dimensional electrophoresis (2-DE) and Western blotting. Proteomic analysis revealed 69 differentially regulated proteins, including such apoptosis-related species as annexins, lamins, and heat shock proteins, which were unanimously upregulated in the SCNT sample. Among the downregulated proteins in SCNT pancreas were peroxiredoxins and catalase. Western blot results indicate that several antioxidant enzymes and the anti-apoptotic protein were downregulated in SCNT pancreas, whereas several caspases were upregulated. Together, these data suggest that the accumulation of reactive oxygen species (ROS) in the pancreas of an adult cloned pig leads to apoptosis. (C) 2007 Elsevier Inc. All rights reserved.