Cryoprotectant Toxicity: Facts, Issues, and Questions.
Cryoprotectant Toxicity: Facts, Issues, and Questions.
复制标题
DOI：
10.1089/rej.2014.1656
复制
发表时间：
2015-10
影响因子：
2.6
通讯作者：
Best BP
中科院分区：
文献类型：
--
作者：
Best BP
高水平的穿透性冷冻保护剂（cpa）可以消除细胞、组织和器官在低温下冷冻保存过程中形成的冰。但随着浓度的增加，注册会计师的毒性也越来越大。人们已经尝试了许多策略来克服在减少毒性的同时消除冰的问题，例如尝试优化冷却和升温速率，或尝试优化在冷却过程中添加单个cpa的时间。由于目前使用的策略不充分，CPA毒性仍然是冷冻保存的最大障碍。CPA的毒性阻碍了人体器官的低温保存，而低温保存有可能挽救许多生命。这篇综述试图描述什么是已知的CPA毒性，理论的CPA毒性和策略，以减少CPA毒性。批判性的分析和建议也包括在内。
High levels of penetrating cryoprotectants (CPAs) can eliminate ice formation during cryopreservation of cells, tissues, and organs to cryogenic temperatures. But CPAs become increasingly toxic as concentration increases. Many strategies have been attempted to overcome the problem of eliminating ice while minimizing toxicity, such as attempting to optimize cooling and warming rates, or attempting to optimize time of adding individual CPAs during cooling. Because strategies currently used are not adequate, CPA toxicity remains the greatest obstacle to cryopreservation. CPA toxicity stands in the way of cryogenic cryopreservation of human organs, a procedure that has the potential to save many lives. This review attempts to describe what is known about CPA toxicity, theories of CPA toxicity, and strategies to reduce CPA toxicity. Critical analysis and suggestions are also included.