MicroRNA-30e reduces cell growth and enhances drug sensitivity to gefitinib in lung carcinoma.
MicroRNA-30e reduces cell growth and enhances drug sensitivity to gefitinib in lung carcinoma.
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DOI：
10.18632/oncotarget.13944
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发表时间：
2017-01-17
期刊：
影响因子：
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通讯作者：
Shen H
中科院分区：
文献类型：
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作者：
Ning ZQ;Lu HL;Chen C;Wang L;Cai W;Li Y;Cao TH;Zhu J;Shu YQ;Shen H
MicroRNAs （miRNAs）在包括恶性肿瘤在内的各种生物过程中发挥着关键作用。在这里，我们证明了与邻近正常组织相比，miR-30e水平在人肺癌标本中显着降低。此外，与PC9细胞相比，耐药PC9/吉非替尼（PC9G）癌细胞中的mir -30含量明显较低。同时，pc9g细胞中mir - 30e过表达导致细胞增殖和迁移减少，逆转对吉非替尼的耐药。相反，PC9细胞中的miR-30e沉默增加了增殖和迁移，并赋予了对吉非替尼的抗性。此外，作为mir -30的新靶点，HOXA1在调节细胞命运、早期发育模式和器官发生中发挥着重要作用。重要的是，mir -30e在体内也抑制PC9G的生长。综上所述，这些发现表明mir -30应该被认为是一种肿瘤抑制miRNA，可以用于治疗人类肺癌。
MicroRNAs (miRNAs) play critical roles in variousbiological processes,including malignancy. Here, we demonstrated that miR-30e levels were markedly reduced in human lung carcinoma specimens in comparisonwith adjacent normal tissues. In addition, miR-30eamounts were starkly lower in the resistant PC9/gefitinib (PC9G) cancer cells compared with PC9 cells. Meanwhile, miR-30eoverexpression inPC9G cells resulted in reduced cell proliferation and migration,reversing drug resistance to gefitinib.Conversely,miR-30e silencing in PC9 cells increased proliferation as well as migration, and conferred resistance to gefitinib.Moreover, HOXA1, which was identified asa new miR-30etarget, plays important roles in regulating cell fate, early developmental patterns and organogenesis.Importantly, miR-30ealso inhibited PC9G growth in vivo. Taken together, these findings demonstrated that miR-30eshould be considered a tumor suppressor miRNA, which could be used in treatinghuman lung cancer.