Transinfection of Wolbachia in Planthoppers: Nymphal Injection of Cultured Wolbachia and Infection Dynamics
Transinfection of Wolbachia in Planthoppers: Nymphal Injection of Cultured Wolbachia and Infection Dynamics
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DOI：
10.1603/022.038.0615
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发表时间：
2009-12-01
影响因子：
1.7
通讯作者：
Noda, Hiroaki
中科院分区：
文献类型：
--
作者：
Kawai, Sawako;Matsumoto, Yukiko;Noda, Hiroaki
沃尔巴克氏体是细胞内共生体，会导致节肢动物的生殖改变。在许多节肢动物物种中进行了转染实验，以阐明沃尔巴克氏体与新宿主之间的相互作用。为了方便这种细菌转染新的节肢动物宿主，我们引入了两种技术：若虫注射代替胚胎注射，以及使用沃尔巴克氏体培养源代替直接从供体转移到受体。将小褐飞虱Laodelphax striatellus中的沃尔巴克氏体在细胞系中培养，并将其与细胞一起注射到褐飞虱Nilaparvata lugens的若虫体腔中。通过使用这些技术，获得了两个转染的飞虱品系。在一个谱系中，沃尔巴克氏体在几代之后就消失了；在另一株系中，沃尔巴克氏体保留了>7年。在转注射后的早期世代中，后一转染品系的感染率约为 80%，但随后下降至
Wolbachia species are intracellular symbionts that cause reproductive alterations in arthropods. Transinfection experiments have been performed in many arthropod species to elucidate the interaction between Wolbachia and a new host. To ease transinfection of this bacterium to new arthropod hosts, we introduced two techniques: nymphal injection instead of embryonic injection and the use of a cultured source of Wolbachia instead of direct transfer from donors to recipients. Wolbachia in the small brown planthopper Laodelphax striatellus was cultivated in a cell line and injected into the nymphal body cavity of the brown planthopper Nilaparvata lugens together with the cells. By using these techniques, two transinfected planthopper lines were obtained. in one line, Wolbachia disappeared after several generations; in the other line, Wolbachia was retained for >7 yr. Infection rates in this latter transinfected line were approximate to 80% in early generations after transinjection but decreased to