Menstrual phase-dependent gene expression differences in periurethral vaginal tissue from women with stress incontinence
Menstrual phase-dependent gene expression differences in periurethral vaginal tissue from women with stress incontinence
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DOI：
10.1067/mob.2003.373
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发表时间：
2003-07-01
影响因子：
9.8
通讯作者：
Polan, ML
中科院分区：
文献类型：
--
作者：
Chen, B;Wen, Y;Polan, ML
目的：泌尿生殖道的机械稳定性依赖于支撑膀胱颈、尿道和盆腔器官的完整胶原纤维。我们推测胶原代谢的遗传差异可能导致压力性尿失禁。由于性激素对女性的下尿路在整个成年生活中有实质性的影响，我们研究了在月经周期的增殖期，与没有压力性尿失禁的女性相比，压力性尿失禁女性阴道组织的基因表达。采用定量竞争性聚合酶链反应技术验证尿道周围阴道组织和骨盆韧带组织中基因表达的相似性组织.标记的互补RNA从五对年龄和月经相匹配的绝经前妇女和无压力性尿失禁的尿道周围阴道组织中获得。然后将阴道组织在含有代表6800个全长人类基因的探针的HuGeneFL阵列上杂交。独立使用Student t检验和Mann-Whitney排名来选择具有概率值的候选者
OBJECTIVE: The mechanical stability of the genitourinary tract is dependent on intact collagen fibers that support the bladder neck, urethra, and pelvic organs. We hypothesize that genetic differences in collagen metabolism may contribute to stress urinary incontinence. Because sex hormones have substantial influence on the female lower urinary tract throughout adult life, we investigated the gene expression of vaginal tissue of women with stress incontinence compared with women with no stress incontinence in the proliferative phase of the menstrual cycle.STUDY DESIGN: Quantitative competitive polymerase chain reaction was used to verify that the gene expressions were similar between periurethral vaginal tissue and pelvic ligamentous tissue. Labeled complementary RNA was obtained from periurethral vaginal tissue in five pairs of age- and menstrual phase-matched, premenopausal women with and without stress urinary incontinence. The vaginal tissues were then hybridized on HuGeneFL arrays that contained probes representing 6800 full-length human genes. The Student t test and Mann-Whitney ranking were used independently to select candidates with probability values