Using Genetically Encoded Fluorescent Biosensors for Quantitative In Vivo Imaging
Using Genetically Encoded Fluorescent Biosensors for Quantitative In Vivo Imaging
复制标题
使用基因编码荧光生物传感器进行体内定量成像
DOI：
10.1007/978-1-0716-0880-7_14
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Nakamura Masayoshi
中科院分区：
文献类型：
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作者：
Yoshinari Akira;Moe-Lange Jacob;Kleist Thomas J.;Cartwright Heather N.;Quint David A.;Ehrhardt David W.;Frommer Wolf B.;Nakamura Masayoshi
荧光生物传感器是用于跟踪活生物体中的分析物或细胞过程并允许可视化细胞调节剂的空间和时间动态的强大工具。基于荧光蛋白的生物传感器由于其高选择性和低侵入性而被广泛应用。各种基于FP的生物传感器已被设计并应用于植物研究中，以可视化pH值，氧化还原状态，分子浓度（离子，糖，肽，ATP，活性氧和植物激素）和转运蛋白活性的动态变化。在这一章中，我们简要总结了报道的使用FP为基础的生物传感器在植物和显示简单的方法来监测细胞内Ca 2 +inArabidopsis thaliana使用比率遗传编码的Ca 2+指示剂，MatryoshCaMP6s的动态。
Fluorescent biosensors are powerful tools for tracking analytes or cellular processes in live organisms and allowing visualization of the spatial and temporal dynamics of cellular regulators. Fluorescent protein (FP)-based biosensors are extensively employed due to their high selectivity and low invasiveness. A variety of FP-based biosensors have been engineered and applied in plant research to visualize dynamic changes in pH, redox state, concentration of molecules (ions, sugars, peptides, ATP, reactive oxygen species, and phytohormones), and activity of transporters. In this chapter, we briefly summarize reported uses of FP-based biosensors in planta and show simple methods to monitor the dynamics of intracellular Ca2+inArabidopsis thalianausing a ratiometric genetically encoded Ca2+indicator, MatryoshCaMP6s.