The effect of nitric oxide synthase inhibitor on form-deprivation myopia
The effect of nitric oxide synthase inhibitor on form-deprivation myopia
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DOI：
10.1076/ceyr.16.10.992.9021
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发表时间：
1997-10-01
影响因子：
2
通讯作者：
Tano, Y
中科院分区：
文献类型：
--
作者：
Fujikado, T;Kawasaki, Y;Tano, Y
目的。形觉剥夺性近视被认为主要是由于调节视网膜信息处理的物质的作用所致。我们使用一个雏鸡模型来研究一氧化氮(NO)，一种气体神经调节剂在FDM的发展中可能起到什么作用。给6日龄雏鸡左眼注射不同浓度的一氧化氮合酶抑制剂N omega-硝基-L-精氨酸甲酯(L-NAME)(30mI)，右眼注射等体积的生理盐水，多数雏鸡用半透明护目镜遮盖双眼6d。摘除护目镜后，用验光镜测量屈光度，用A型超声测量眼轴长度。在一些雏鸡中，我们测量了视网膜中NOx(亚硝酸盐和硝酸盐)的浓度。结果在戴封堵器的雏鸡中，注射L NAME(180 mm、360 mm、540 mm)的雏鸡的屈光不正和眼轴长度的影响显著低于对照眼(右侧)。ERG改变是可逆的，除了注射最高浓度(540 MM)的L-NAME眼。注射L的雏鸡，在无遮盖的情况下饲养，眼睛的屈光度正常。在形觉剥夺6d后，注射L-NAME(180 MM)的眼视网膜中NOx浓度明显低于注射生理盐水的眼。发育期雏鸡遮盖前注射L可引起视网膜功能的可逆性改变，抑制形觉剥夺性近视的发展。
Purpose. Form-deprivation myopia (FDM) is believed to result principally from actions of substances that modulate information processing in the retina. We used a chick model to investigate what role nitric oxide (NO), a gaseous neuromodulator might play in the development of FDM.Methods. We injected different concentrations of the NO synthase inhibitor N omega-nitro-L-arginine methyl ester (L-NAME) (30 mi) into the left eyes and the same volume of saline into the right eyes of 6-day-old chicks, Both eyes of most chicks were occluded for 6 days with translucent goggles. After removal of the goggles, the refraction was measured by retinoscopy and the axial lengths with an A-mode ultrasound. In some chicks we measured the concentration of NOx (nitrite and nitrate) in the retina. A few chicks, not wearing occluders after injection of L-NAME and saline, ERG and refraction, were examined 6 days after the treatment.Results, In chicks that wore occluders, refractive error and axial length were significantly less affected in eyes injected with L-NAME (180, 360, or 540 mM) compared to control (right) eyes. ERG changes were reversible, except in eyes injected with the highest concentration (540 mM) of L-NAME. The eyes of chicks, Injected with L-NAME and reared without occlusion, had normal refractive values. After 6 days of form deprivation, the concentration of NOx in the retina of eves injected with L-NAME (180 mM) was significantly less than the concentration in eyes injected with saline.Conclusions. The injection of L-NAME before occlusion of developing chick, eyes leads to reversible modifications in retinal function and inhibits the development of form-deprivation myopia.