Transcription factor genes essential for cell proliferation and replicative lifespan in budding yeast.
Transcription factor genes essential for cell proliferation and replicative lifespan in budding yeast.
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DOI：
10.1016/j.bbrc.2015.05.067
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发表时间：
2015-07
影响因子：
3.1
通讯作者：
Yuka Kamei;A. Tai;Shota Dakeyama;Kaori Yamamoto;Yamato Inoue;Yoshifumi Kishimoto;Hiroya Ohara;Y. Mukai
中科院分区：
文献类型：
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作者：
Yuka Kamei;A. Tai;Shota Dakeyama;Kaori Yamamoto;Yamato Inoue;Yoshifumi Kishimoto;Hiroya Ohara;Y. Mukai
许多与寿命相关的基因已经在从酵母到人类的真核生物中被鉴定出来。然而，关于细胞增殖所必需的长寿基因的信息有限。在这里，我们研究了编码DNA结合转录因子的必需基因是否调节酿酒酵母的复制寿命。FHL 1、RAP 1、REB 1和MCM 1基因杂合二倍体敲除菌株寿命明显缩短，1H-核磁共振分析表明Δ fhl 1/FHL 1突变体具有特征性的代谢特征。这些结果有力地表明FHL 1调节寿命相关代谢基因的转录。因此，杂合敲除菌株可能是发现新的寿命基因的潜在材料。
Many of the lifespan-related genes have been identified in eukaryotes ranging from the yeast to human. However, there is limited information available on the longevity genes that are essential for cell proliferation. Here, we investigated whether the essential genes encoding DNA-binding transcription factors modulated the replicative lifespan ofSaccharomyces cerevisiae. Heterozygous diploid knockout strains forFHL1, RAP1,REB1, andMCM1genes showed significantly short lifespan.1H-nuclear magnetic resonance analysis indicated a characteristic metabolic profile in the Δfhl1/FHL1mutant. These results strongly suggest thatFHL1regulates the transcription of lifespan related metabolic genes. Thus, heterozygous knockout strains could be the potential materials for discovering further novel lifespan genes.