HIGH-ENERGY PHOSPHATE CONCENTRATIONS IN DOG MYOCARDIUM DURING STRESS
HIGH-ENERGY PHOSPHATE CONCENTRATIONS IN DOG MYOCARDIUM DURING STRESS
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DOI：
10.1152/ajplegacy.1969.216.5.1103
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发表时间：
1969-01-01
影响因子：
--
通讯作者：
POOL, PE
中科院分区：
文献类型：
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作者：
BOERTH, RC;COVELL, JW;POOL, PE
方法6只杂种犬静脉注射戊巴比妥钠（平均37 mg/kg）麻醉。左心室等容准备在之前已经详细介绍过(5)，如图1所示。静脉血从双腔静脉中抽出，充氧后逆行泵入股动脉。结扎肺动脉，打开左心房，切除腱索和二尖瓣。通过二尖瓣环将薄乳胶球囊与宽口径金属套管连接在一起，置于左心室。球囊通过左心室流出道和二尖瓣环内的塑料塞维持在左心室内。通过金属套管向球囊内注入一定量的生理盐水以调节心室容积。心脏通过电从右心房开始跳动。将Statham P23Db换能器连接在锁骨下动脉插管上测量全身动脉压，将类似的换能器连接在左心室金属套管上测量左心室压。用模拟计算机（Electronic Associates, Inc., Long Branch， NJ；型号TR-20）连续计算壁面应力，公式为：应力= Pr/Zh，其中P= LVP（单位为g/cm2）， r=内半径（单位为cm）， h=壁厚（单位为cm）(5)。在a上记录全身动脉压、LVP、LVP对时间的一阶导数（LV dP/dt）和心肌壁应力
METHODSSix mongrel dogs were anesthetized with intravenous pentobarbital sodium (avg 37 mg/kg). The left ventricular isovolumic preparation has been presented in detail previously (5), and is shown schematically in Fig. 1. Venous blood was drained from both venae cavae, oxygenated, and pumped retrogradely into a femoral artery. The pulmonary artery was ligated, the left atrium opened, and the chordae tendineae and mitral valve excised. A thin latex balloon attached to a wide-bore metal cannula was placed in the left ventricle through the mitral annulus. The balloon was maintained within the left ventricle by plastic plugs in the left ventricular outflow tract and in the mitral annulus. Known amounts of saline were introduced into the balloon through the metal cannula to regulate ventricular volume. The heart was electrically paced from the right atrium. Systemic arterial pressure was measured with a Statham P23Db transducer attached to a cannula in the subclavian artery, and left ventricular presssure (LVP) was measured with a similar transducer attached to the metal cannula in the left ventricle. Wall stress was continuously calculated with an analog computer (Electronic Associates, Inc., Long Branch, NJ; model TR-20) according to the equation: stress= Pr/Zh, where P= LVP (in g/cm2), r= internal radius (in cm), and h= wall thickness (in cm)(5). Systemic arterial pressure, LVP, the first derivative of LVP with respect to time (LV dP/dt), and myocardial wall stress were recorded on a