Ten new microsatellite loci for analysis of genetic diversity in isolated populations of the Alpine land snail Cylindrus obtusus
Ten new microsatellite loci for analysis of genetic diversity in isolated populations of the Alpine land snail Cylindrus obtusus
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DOI：
10.1007/s10592-009-9893-1
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发表时间：
2010-06-01
影响因子：
2.2
通讯作者：
Kruckenhauser, Luise
中科院分区：
文献类型：
--
作者：
Arthofer, Wolfgang;Cadahia, Luis;Kruckenhauser, Luise
在这项研究中，我们对奥地利阿尔卑斯山特有的陆地钉螺Cylindrus obtusus的10个微卫星标记进行了分析，这些标记分布在海拔约1600m以上的孤立种群中。每个座位的等位基因数在2到8个之间。观察杂合度在0.00~1.00之间，期望杂合度在0.09~0.72之间。两个基因座之间没有发现显著的连锁不平衡。其中一个群体(Dachstein)没有偏离Hardy-Weinberg平衡，也不存在零等位基因，而另一个群体(Schneberg)则显示出零等位基因的存在。这些不同的结果可能反映了群体结构的差异，而不是微卫星座位的特征，并强调了这些标记在研究钝顶梭鱼的遗传多样性、群体结构和分化方面的有效性。
In this study we characterized 10 polymorphic microsatellite markers for the land snail Cylindrus obtusus, an endemism of the Austrian Alps with a distribution in isolated populations above approximately 1,600 m. The microsatellite loci were analyzed in 44 individuals from two populations. Number of alleles per locus ranged between two and eight. Observed heterozygosity ranged between 0.00 and 1.00, and expected heterozygosity between 0.09 and 0.72. No significant linkage disequilibrium was found between pairs of loci. One of the sampled populations (Dachstein) showed no deviation from Hardy-Weinberg equilibrium and no presence of null alleles, whereas the other one (Schneeberg) did. These diverging results probably reflect differences in population structure rather than characteristics of the microsatellite loci and underline the usefulness of these markers for studying genetic diversity, population structure and differentiation in C. obtusus.