Feasibility of Using the Mosquito Blood Meal for Rapid and Efficient Human and Animal Virus Surveillance and Discovery.
Feasibility of Using the Mosquito Blood Meal for Rapid and Efficient Human and Animal Virus Surveillance and Discovery.
复制标题
使用蚊血粉快速有效地监测和发现人类和动物病毒的可行性。
DOI：
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发表时间：
2015
影响因子：
3.3
通讯作者：
R. Jarman
中科院分区：
文献类型：
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作者：
Yu Yang;L. Garver;Karen M Bingham;J. Hang;R. Jochim;S. Davidson;J. Richardson;R. Jarman
从人和动物身上采集的蚊血粉可能是检测血液传播病原体的有用血液来源。在这项可行性研究中，斯氏按蚊用添加了登革2型病毒(DENV-2)的血粉喂养蚊子，并在连续的时间点上收获。这些蚊子不是合格的媒介，病毒预计不会复制。摄入的血液被发现在Whatman FTA卡上，并保存在室温下。取出蚊子腹部，在-80°C下保存。对照血粉等份保存在小瓶中或涂在FTA卡上。储存4周后，用串珠和QIAamp病毒RNA试剂盒(Chigen Sciences，Germantown，MD)提取样品。回收的病毒RNA用DENV-2 TaqMan RT-PCR和下一代测序(NGS)进行分析。从直接使用的干血斑中回收病毒RNA的总体效率为15%，而通过在FTA卡片上印迹蚊子中肠制成的干血斑的总病毒RNA回收率约为20%或更高。蚊子吸入的血液中的病毒RNA会随着时间的推移而减少，但在吸血24小时后仍可检测到。保存在FTA中的样本中的病毒序列可以在室温下保持。该策略在加快人畜共患病病毒发现和血源性病原体监测方面具有潜在的实用价值。
Mosquito blood meals taken from humans and animals potentially represent a useful source of blood for the detection of blood-borne pathogens. In this feasibility study, Anopheles stephensi mosquitoes were fed with blood meals spiked with dengue virus type 2 (DENV-2) and harvested at serial time points. These mosquitoes are not competent vectors, and the virus is not expected to replicate. Ingested blood was spotted on Whatman FTA cards and stored at room temperature. Mosquito abdomens were removed and stored at -80°C. Control blood meal aliquots were stored in vials or applied onto FTA cards. After 4 weeks of storage, the samples were extracted using beadbeating and QIAamp Viral RNA kit (Qiagen Sciences, Germantown, MD). Recovered viral RNA was analyzed by DENV-2 TaqMan RT-PCR assay and next-generation sequencing (NGS). Overall viral RNA recovery efficiency was 15% from the directly applied dried blood spots and approximately 20% or higher for dried blood spots made by blotting mosquito midgut on FTA cards. Viral RNA in mosquito-ingested blood decreases over time, but remains detectable 24 hours after blood feeding. The viral sequences in FTA-stored specimens can be maintained at room temperature. The strategy has the potential utility in expedited zoonotic virus discovery and blood-borne pathogen surveillance.
DOI：
10.1056/nejmoa1401268
发表时间：
2014-06-19
期刊：
The New England journal of medicine
影响因子：
--
作者：
Wilson MR;Naccache SN;Samayoa E;Biagtan M;Bashir H;Yu G;Salamat SM;Somasekar S;Federman S;Miller S;Sokolic R;Garabedian E;Candotti F;Buckley RH;Reed KD;Meyer TL;Seroogy CM;Galloway R;Henderson SL;Gern JE;DeRisi JL;Chiu CY
通讯作者：
Chiu CY