Impact of nutrition on postnatal development of serine-threonine dehydratase and branched-chain keto acid dehydrogenase in the rat.
Impact of nutrition on postnatal development of serine-threonine dehydratase and branched-chain keto acid dehydrogenase in the rat.
复制标题
营养对大鼠丝氨酸苏氨酸脱水酶和支链酮酸脱氢酶产后发育的影响。
DOI：
10.1159/000242856
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
M. Picciano
中科院分区：
文献类型：
--
作者：
C. K. Grogan;L. Janas;M. K. Hendrix;D. Layman;M. Picciano
本文研究了生长期大鼠两种氨基酸分解代谢酶丝氨酸-苏氨酸脱氢酶（STD）和支链α-酮酸脱氢酶（BCKAD）的发育模式。在10，15，20，30和60日龄，STD和BCKAD活性测定幼仔组织。在10至20日龄之间，肝脏STD活性增加了3倍以上;在此峰值之后，活性在30日龄时下降。三次给药不影响任何年龄的STD活动。在肝脏、肾脏、大脑和骨骼肌中，总BCKAD活性在10至30日龄之间增加2至4倍。在10至15日龄之间，所有组织中的BCKAD活性降低，与总活性的大幅增加相关。BCKAD活性的百分比在20日龄至30日龄之间没有变化，但从30日龄至60日龄增加，与总活性的降低相关。亮氨酸给药不影响任何年龄的总BCKAD活性。这些结果表明，STD和BCKAD在哺乳期发展较晚，总活动对过量的膳食氨基酸无反应。
The developmental patterns of two amino acid-catabolizing enzymes, serine-threonine dehydratase (STD) and branched-chain alpha-keto acid dehydrogenase (BCKAD), were investigated in growing rats. At 10, 15, 20, 30, and 60 days of age, STD and BCKAD activities were determined in pup tissues. Hepatic STD activity increased more than 3-fold between 10 and 20 days of age; after this peak, activity decreased by 30 days of age. Threonine dosing did not affect STD activity at any age. In the liver, kidney, brain and skeletal muscle, total BCKAD activity increased 2- to 4-fold between 10 and 30 days of age. Percentages of BCKAD active in all tissues decreased between 10 and 15 days of age, associated with a large increase in total activity. The percentages of BCKAD activity were unchanged between 20 and 30 days of age but increased from 30 to 60 days of age, associated with a decrease in total activity. Leucine dosing did not affect total BCKAD activity at any age. These results demonstrate that both STD and BCKAD develop late during the suckling period and total activities are unresponsive to excess dietary amino acids.