Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer‐binding factor mediates specific induction of virus immediate‐early gene expression.
Direct combinatorial interaction between a herpes simplex virus regulatory protein and a cellular octamer‐binding factor mediates specific induction of virus immediate‐early gene expression.
复制标题
单纯疱疹病毒调节蛋白和细胞八聚体结合因子之间的直接组合相互作用介导病毒立即早期基因表达的特异性诱导。
作者：
P. O’Hare;C. Goding;A. Haigh
We provide evidence for a novel mechanism of transcriptional regulation in which the immediate‐early (IE) transactivating protein of herpes simplex virus, Vmw65, is assembled into a specific DNA‐binding complex together with a cellular octamer‐binding factor (TRF). The assembly of Vmw65/TRF complex requires not only the core TRF recognition site, but also flanking sequences which are dispensable for TRF binding alone. We show from functional analyses that TRF binding by a motif is required but not sufficient to confer induction on a heterologous promoter, and it is the ability of the motif to allow TRF/Vmw65 complex assembly which correlates with functional activity. Thus, for the induction of HSV IE expression, Vmw65 forms a complex with TRF by recognition of the specific subset of appropriately flanked TRF binding sites present in each of the IE genes. This mechanism may provide a paradigm for the selective utilization of the same transcription factor in differential gene expression.
