DECONSTRUCTING THE PERINEURONAL NET: CELLULAR CONTRIBUTIONS AND MOLECULAR COMPOSITION OF THE NEURONAL EXTRACELLULAR MATRIX
DECONSTRUCTING THE PERINEURONAL NET: CELLULAR CONTRIBUTIONS AND MOLECULAR COMPOSITION OF THE NEURONAL EXTRACELLULAR MATRIX
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DOI：
10.1016/j.neuroscience.2012.05.055
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发表时间：
2012-08-30
期刊：
影响因子：
3.3
通讯作者：
Matthews, R. T.
中科院分区：
文献类型：
--
作者：
Giamanco, K. A.;Matthews, R. T.
神经元束膜网（perineuronalnets，PNNs）是神经细胞外基质的格子状亚结构，包裹着整个中枢神经系统中的特定神经元群体。以前的工作表明，这种结构在调节发育神经可塑性和大脑成熟中起着重要作用。理解这些结构的确切作用受到阻碍，不完全理解其分子组成和细胞的贡献，其形成，这是研究在这里使用原代皮层细胞培养。通过定义减少（添加胞苷-β-D-阿拉伯呋喃糖苷/AraC）或几乎消除（增加氯化钾（KCl）和AraC应用）胶质细胞的培养条件，鉴定了受该细胞类型影响的PNN成分。还评估了单独去极化KCl浓度的影响。我们的工作确定了聚集蛋白聚糖作为PNN的主要神经元成分，其表达通过去极化和胶质细胞抑制显著上调，此外，聚集蛋白聚糖阳性PNN的发展加速。令人惊讶的是，在我们的培养系统中，大多数测试的其他PNN组分都是以神经胶质依赖的方式制备的。有趣的是，在没有这些神经胶质衍生成分的情况下，形成了聚集蛋白聚糖和透明质酸反应性PNN，表明这两种成分足以进行基础PNN组装。其他成分以胶质依赖的方式表达。总的来说，这项工作提供了更深入的了解神经元和神经胶质细胞之间的复杂的相互作用，在PNN的形成，并提高了我们对这些结构的分子组成的理解。(C)2012年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Perineuronal nets (PNNs) are lattice-like substructures of the neural extracellular matrix that enwrap particular populations of neurons throughout the central nervous system. Previous work suggests that this structure plays a major role in modulating developmental neural plasticity and brain maturation. Understanding the precise role of these structures has been hampered by incomplete comprehension of their molecular composition and cellular contributions to their formation, which is studied herein using primary cortical cell cultures. By defining culture conditions to reduce (cytosine-beta-D-arabinofuranoside/AraC addition) or virtually eliminate (elevated potassium chloride (KCl) and AraC application) glia, PNN components impacted by this cell type were identified. Effects of depolarizing KCl concentrations alone were also assessed. Our work identified aggrecan as the primary neuronal component of the PNN and its expression was dramatically up-regulated by both depolarization and glial cell inhibition and additionally, the development of aggrecan-positive PNNs was accelerated. Surprisingly, most of the other PNN components tested were made in a glial-dependent manner in our culture system. Interestingly, in the absence of these glial-derived components, an aggrecan-and hyaluronan-reactive PNN developed, demonstrating that these two components are sufficient for base PNN assembly. Other components were expressed in a glial-dependent manner. Overall, this work provides deeper insight into the complex interplay between neurons and glia in the formation of the PNN and improves our understanding of the molecular composition of these structures. (C) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.