Light-induced root hair formation in lettuce (Lactuca sativa L. cv. Grand Rapids) roots at low pH is brought by chlorogenic acid synthesis and sugar
Light-induced root hair formation in lettuce (Lactuca sativa L. cv. Grand Rapids) roots at low pH is brought by chlorogenic acid synthesis and sugar
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DOI：
10.1007/s10265-010-0328-3
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发表时间：
2010-11-01
影响因子：
2.8
通讯作者：
Inoue, Yasunori
中科院分区：
文献类型：
--
作者：
Narukawa, Megumi;Watanabe, Keiji;Inoue, Yasunori
在此之前，我们报道了绿原酸（CGA）促进莴苣（Lactucasativa L.）在pH 4.0时根毛的形成。CV.大急流城）。光对这个过程至关重要。在本研究中，我们确定了生菜幼苗根毛形成过程中CGA，光和糖之间的关系。在完整幼苗中，白色光下CGA含量增加。外源施加的CGA恢复根毛形成在黑暗中生长的完整幼苗在pH 4.0。然而，没有根毛的形成诱导在断头苗无论光照和CGA应用。蔗糖或葡萄糖的应用诱导根毛的形成和CGA的合成在光生长断头苗在pH 4.0。蓝光是最有效的根毛的形成和CGA合成时，供应蔗糖去头幼苗。此外，蔗糖和CGA一起诱导根毛形成在pH 4.0的黑暗生长的断头苗。结果表明，光诱导CGA的合成从根中的糖。除了CGA合成的起始材料之外，糖也是根毛形成所必需的。此外，一个未知的低pH诱导因子是生菜根毛形成所必需的。
Previously, we reported that chlorogenic acid (CGA) facilitated root hair formation at pH 4.0 in lettuce (Lactuca sativa L. cv. Grand Rapids). Light was essential for this process. In the present study, we determined relationships between CGA, light, and sugar during root hair formation in lettuce seedlings. The amount of CGA increased with white light in intact seedlings. Exogenously applied CGA restored root hair formation in dark-grown intact seedlings at pH 4.0. However, no root hair formation was induced in decapitated seedlings regardless of light exposure and CGA application. Application of sucrose or glucose induced both root hair formation and CGA synthesis in light-grown decapitated seedlings at pH 4.0. Blue light was the most effective for both root hair formation and CGA synthesis when supplied with sucrose to decapitated seedlings. Addition of sucrose and CGA together induced root hair formation at pH 4.0 in dark-grown decapitated seedlings. Results suggest that light induced CGA synthesis from sugar in the roots. Sugar was also required for root hair formation other than starting material of CGA synthesis. In addition, an unknown low pH-induced factor was essential for lettuce root hair formation.