p53 nuclear accumulation and multiploidy are adverse prognostic factors in surgically resected stage II colorectal cancers independent of fluorouracil-based adjuvant therapy
p53 nuclear accumulation and multiploidy are adverse prognostic factors in surgically resected stage II colorectal cancers independent of fluorouracil-based adjuvant therapy
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DOI：
10.1309/v7uw-ut2e-jvyh-dgwk
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发表时间：
2001-09-01
影响因子：
3.5
通讯作者：
Mottolese, M
中科院分区：
文献类型：
--
作者：
Buglioni, S;D'Agnano, I;Mottolese, M
为了确定最高风险的患者，用单克隆抗体DO 7和多克隆抗体CM 1测定了94例手术切除的II期患者的DNA含量和p53细胞核或细胞质积聚（Dukes B2）结直肠癌，接受或未接受基于5-氟尿嘧啶的辅助化疗。61例（65%）肿瘤为异倍体，其中16例（17%）有多倍体DNA含量，50例（53%）显示DO 7核p53积聚，44例（47%）显示胞浆CM 1阳性。在多变量分析中，只有多倍性和p53核阳性是预后较差的独立预后指标。在5-氟尿嘧啶治疗和未治疗的患者中，p53阳性与较短的生存期相关，因此，在Dukes B2结直肠癌患者中，基于DNA多倍性和p53状态联合评估的生物学特征可以提供有价值的预后信息。确定患者参加替代的，更积极的治疗试验。
To identify the prognostically highest risk patients, DNA content and p53 nuclear or cytoplastmic accumulation, evaluated by monoclonal antibody DO7 and polyclonal antibody CM1, were determined in 94 surgically resected stage II (Dukes B2) colorectal cancers, treated or not with adjuvant 5-fluorouracil-based chemotherapy.Sixty-one (65%) of the tumors were aneuploid, 16 (17%) of which had a multiploid DNA content; 50 (53%) displayed DO7 nuclear p53 accumulation, and 44 (47%) showed cytoplasmic CM1 positivity. In multivariate analysis, only multiploidy and p53 nuclear positivity emerged as independent prognostic indicators of a poorer outcome. Positivity, for p53 was associated with shorter survival in 5-fluorouracil-treated and untreated patients.Therefore, in patients with Dukes B2 colorectal cancer a biologic profile based on the combined evaluation of DNA multiploidy and p53 status can provide valuable prognostic information. identifying patients to be enrolled in alternative, more aggressive therapeutic trials.