Myosin-II-mediated cell shape changes and cell intercalation contribute to primitive streak formation.
Myosin-II-mediated cell shape changes and cell intercalation contribute to primitive streak formation.
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DOI：
10.1038/ncb3138
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发表时间：
2015-04
影响因子：
21.3
通讯作者：
Weijer CJ
中科院分区：
文献类型：
--
作者：
Rozbicki E;Chuai M;Karjalainen AI;Song F;Sang HM;Martin R;Knölker HJ;MacDonald MP;Weijer CJ
鸡胚原条的形成涉及上胚层中超过100.000个细胞的大规模高度协调的流动。这些大规模的组织流动和变形可以通过组合的光片显微镜和计算分析与形成中内胚层中的特定各向异性细胞行为相关。相关的行为包括顶端收缩，沿沿着顶端-基底轴伸长，然后向内移动以及小群中内胚层细胞的异步定向细胞嵌入。细胞嵌入与顶端连接的顺序、定向收缩相关，其发生、定位和方向与相邻细胞中对齐的顶端连接中活性肌球蛋白II电缆的出现密切相关。一类特异性肌球蛋白抑制剂和基因特异性敲除的使用表明，顶端收缩和嵌入是肌球蛋白II依赖性的，也揭示了肌球蛋白I和肌球蛋白V家族成员在连接肌球蛋白II电缆的组装中的关键作用。
Primitive streak formation in the chick embryo involves large scale highly coordinated flows of over 100.000 cells in the epiblast. These large scale tissue flows and deformations can be correlated with specific anisotropic cell behaviours in the forming mesendoderm through a combined light-sheet microscopy and computational analysis. Relevant behaviours include apical contraction, elongation along the apical-basal axis followed by ingression as well as asynchronous directional cell intercalation of small groups of mesendoderm cells. Cell intercalation is associated with sequential, directional contraction of apical junctions, the onset, localisation and direction of which correlate strongly with the appearance of active Myosin II cables in aligned apical junctions in neighbouring cells. Use of a class specific Myosin inhibitors and gene specific knockdowns show that apical contraction and intercalation are Myosin II dependent and also reveal critical roles for Myosin I and Myosin V family members in the assembly of junctional Myosin II cables.