Diagnostic and prognostic relevance of expression of human telomerase subunits in oral cancer.
Diagnostic and prognostic relevance of expression of human telomerase subunits in oral cancer.
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口腔癌中人端粒酶亚基表达的诊断和预后相关性。
DOI：
10.3892/ijo.19.5.1063
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发表时间：
2001
影响因子：
5.2
通讯作者：
J. Ries
中科院分区：
文献类型：
--
作者：
B. K. Lee;E Diebel;F. Neukam;J. Wiltfang;J. Ries
端粒酶活性(TA)与大多数人类恶性肿瘤有关，但在正常体细胞中检测不到，只有少数例外。端粒酶的三个主要亚基(hTR、hTP1和hTERT)已被鉴定。为探讨TA、hTR、hTP1和hTERT在口腔癌诊断和预后判断中的临床意义，我们采用高灵敏度的TRAP法和RT-PCR法检测了46例口腔鳞癌和15例正常口腔黏膜组织中TA、hTR、hTP1和hTERT的表达。无论TA表达与否，所有标本均检测到hTR和hTP1基因的表达。反之，hTERT表达与TA显著相关，提示端粒酶活性受hTERT转录调控。此外，hTERT的表达与肿瘤的发生密切相关。正常粘膜组织中无一例表达hTERT亚单位，而肿瘤组织中有76%(35/46)表达。其他临床病理指标和预后指标均与TA或hTERT表达无明显相关性。这些结果提示，检测hTERT的表达可能是另一种有用的诊断指标，特别是在口腔鳞癌的早期诊断和鉴别健康组织和肿瘤转化组织方面。
Telomerase activity (TA) is associated with most malignant human tumors but is not detected in normal somatic cells with a few exceptions. Three major subunits (hTR, hTP1 and hTERT) of telomerase have been identified. To investigate the clinical implications of the mRNA detection of these components as useful diagnostic and prognostic markers in oral cancer, we examined TA, hTR, hTP1 and hTERT mRNA expressions in 46 oral squamous cell carcinomas (OSCC) and 15 normal oral mucosal tissues from healthy volunteers using a highly sensitive TRAP assay and RT PCR. In all specimens hTR and hTP1 mRNA were detected regardless whether TA was expressed or not. On the contrary, a significant correlation between hTERT expression and TA was shown indicating that the activity of telomerase could be regulated by the extent of hTERT transcription. In addition, hTERT expression showed close association to malignancies. None of the normal mucosal specimens expressed the hTERT subunit, but 76% (35/46) of the tumor specimens did. None of other clinico-pathological and prognostic parameters showed significant relationship with TA or hTERT expression. These results suggest that the detection of hTERT expression may be another useful diagnostic marker, especially for early detection of OSCC, and for distinguishing healthy tissues from neoplastically transformed ones.