Interleukin-13 inhibits cell proliferation and stimulates Interleukin-6 formation in isolated human osteoblasts
Interleukin-13 inhibits cell proliferation and stimulates Interleukin-6 formation in isolated human osteoblasts
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DOI：
10.1210/jc.83.9.3285
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发表时间：
1998-09-01
影响因子：
5.8
通讯作者：
Ljunggren, Ö
中科院分区：
文献类型：
--
作者：
Frost, A;Jonsson, KB;Ljunggren, Ö
白细胞介素-13 （IL-13）是最近发现的一种细胞因子，由活化的T细胞分泌并调节炎症反应。我们研究了IL-13对分离的人成骨细胞样细胞（hOB）的影响。IL-13剂量依赖性（1-100 pmol/L）可使[H-3]胸苷在hOB细胞中的掺入率降低50%以上。利用细胞代谢测定和直接细胞计数，我们发现用IL-13处理导致hOB细胞数量减少。这种影响具有时间和剂量依赖性，培养12天后，0.1 nmol/L的IL-13可使细胞数量减少70%。此外，通过核糖核酸酶保护实验，IL-13增加了hOBs中IL-6信使核糖核酸的水平，并刺激IL-6分泌到培养上清中。综上所述，IL-13抑制了人成骨细胞的细胞增殖，并增加了IL-6的形成。我们的研究结果表明，IL-13可能由于成骨细胞生长受损和il -6诱导的破骨细胞募集而导致骨丢失。
Interleukin-13 (IL-13) is a recently identified cytokine that is secreted by activated T cells and regulates inflammatory responses. We have investigated the effects of IL-13 on isolated human osteoblastlike cells (hOB). IL-13 dose-dependently (1-100 pmol/L) reduced the incorporation rate of [H-3]thymidine in hOB cells by more than 50%. Using a cell metabolic assay as well as direct cell counting, we found that treatment with IL-13 lead to a decrease in hOB cell number. The effect was both time and dose dependent, and after 12 days of culture, treatment with IL-13 (0.1 nmol/L) caused a 70% decrease in the number of cells. Also, IL-13 increased the levels of IL-6 messenger ribonucleic acid in hOBs, as measured by ribonuclease protection assay, and stimulated secretion of IL-6 into culture supernatants. In conclusion, IL-13 inhibits cell proliferation and increases IL-6 formation in human osteoblasts. Our findings suggest that IL-13 may cause bone loss due to impaired osteoblastic growth and IL-6-induced osteoclast recruitment.