PLASMID ANALYSIS OF BORRELIA-BURGDORFERI, THE LYME-DISEASE AGENT
PLASMID ANALYSIS OF BORRELIA-BURGDORFERI, THE LYME-DISEASE AGENT
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DOI：
10.1128/jcm.26.3.475-478.1988
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发表时间：
1988-03-01
影响因子：
9.4
通讯作者：
BARBOUR, AG
中科院分区：
文献类型：
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作者：
BARBOUR, AG
对13株莱姆病病原体伯氏疏螺旋体进行质粒分析时，采用了从疏螺旋体中提取质粒富集DNA的简单方法。提取的DNA进行低百分比琼脂糖凝胶电泳，并直接用溴化乙啶染色或将质粒与编码主要外膜蛋白OspA基因的DNA探针原位杂交后进行检测。每个分离物有4到7个不同大小的可识别质粒。13株菌株中只有2株具有相同的质粒谱。ospA基因探针与来自北美和欧洲的菌株的大质粒杂交。经过多次传代的菌株失去了最初存在的六个质粒中的两个。这些发现表明质粒分析作为菌株分型程序和鉴定可能的质粒赋予的毒力因子的潜在用途。
A simple procedure for extraction of plasmid-enriched DNA from borreliae was used in a plasmid analysis of 13 strains of the Lyme disease agent, Borrelia burgdorferi. The extracted DNA was subjected to low-percentage agarose gel electrophoresis and examined either directly by ethidium bromide staining or after hybridization of the plasmids in situ with a DNA probe for the gene encoding the major outer membrane protein OspA. Each isolate had four to seven discernible plasmids of various sizes. Only 2 of the 13 strains had the same plasmid profile. The ospA gene probe hybridized to large plasmids to strains from both North America and Europe. A strain which had been passaged many times was found to have lost two of the six plasmids originally present. These findings indicate the potential usefulness of plasmid analysis as a strain-typing procedure and for identifying possible plasmid-conferred virulence factors.