The characterization of optimal selenized garlic polysaccharides and its immune and antioxidant activity in chickens
The characterization of optimal selenized garlic polysaccharides and its immune and antioxidant activity in chickens
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DOI：
10.1016/j.ijbiomac.2021.03.197
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发表时间：
2021-04-10
影响因子：
8.2
通讯作者：
Li, Jingui
中科院分区：
文献类型：
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作者：
Bo, Ruonan;Ji, Xun;Li, Jingui
本研究旨在优化硒化大蒜多糖（sGPS）的改性条件，并研究其结构表征、免疫和抗氧化活性。本文采用硝酸钠硒化法制备硒化大蒜多糖（sGPS）。然后通过L9（34）正交试验对sGPS的改性条件进行优化。通过傅里叶变换红外（FT-IR）、固体核磁共振（NMR）、x射线衍射（XRD）和热重（TGA）对sGPS的结构进行了表征。采用扫描电镜（SEM）和透射电镜（TEM）观察了sGPS的形貌。体内研究表明，与疫苗免疫对照组相比，sGPS显著提高了血清抗新城疫病毒血凝抑制（HI）抗体滴度，提高了空肠和气管灌洗液分泌IgA （sIgA）、ifn - γ、IL-2的分泌。结果表明，sGPS对鸡肝脏抗氧化活性有一定影响。综上所述，大蒜多糖的最佳改性条件为：反应温度为70℃，Na2SeO3用量为400 mg，反应时间为6 h。硒化修饰大蒜多糖（GPS）可提高其对鸡的免疫和抗氧化活性。(c) 2021 Elsevier B.V.版权所有
The purpose of this study was to optimize modification conditions of selenized garlic polysaccharides (sGPS) and investigate its structural characterization, immune and antioxidant activities. Herein, selenized garlic polysaccharides (sGPS) were prepared using by HNO3-Na2SeO3 selenylation method. And then modification conditions of sGPS were optimized through L9 (34) orthogonal test. The structural characterization of sGPS were identified by the Fourier-transform infrared (FT-IR), Solid-State nuclear magnetic resonance (NMR) spectra, X-ray diffraction (XRD) and thermogravimetric (TGA). The morphology of sGPS was detected using scanning electron microscope (SEM) and transmission electron microscope (TEM). In vivo investigation showed that sGPS significantly improved serum hemagglutination-inhibition (HI) antibody titers against Newcastle disease virus, enhanced secretory IgA (sIgA), IFN-gamma, IL-2 secretion in jejunum and trachea irrigation compared with vaccine immunized control group. Furthermore, it showed that sGPS had some effects on the antioxidant activities in livers of chickens. In conclusion, the optimal modification conditions of sGPS were as follows: reaction temperature was 70 degrees C, the dosage of Na2SeO3 was 400 mg and reaction time was 6 h. The selenylation modification of garlic polysaccharides (GPS) could improve its immune and antioxidant activity in chickens. (c) 2021 Elsevier B.V. All rights reserved.