EIF3D promoted cervical carcinoma through Warburg effect by interacting with GRP78
EIF3D promoted cervical carcinoma through Warburg effect by interacting with GRP78
复制标题
EIF3D通过与GRP78相互作用，通过Warburg效应促进宫颈癌发生
DOI：
10.1080/01443615.2022.2130200
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发表时间：
2022-10-19
影响因子：
1.3
通讯作者：
Qin，Juan
中科院分区：
文献类型：
--
作者：
Liu，Qing;Liu，Jing;Qin，Juan
宫颈癌的发病率在全球女性肿瘤中排名第三，在发展中国家排名第二。然而，真核翻译起始因子3亚单位D （EIF3D）在宫颈癌中的作用尚不清楚。本研究通过体内和体外模型研究了EIF3D对宫颈癌细胞进展的影响及其潜在机制。宫颈癌患者EIF3D表达mRNA及蛋白表达水平升高。EIF3D低表达子宫颈癌患者的无病生存期（DFS）和总生存期（OS）高于EIF3D高表达子宫颈癌患者。与宫颈正常细胞系（UVECs）相比，宫颈癌细胞系（AV3、Hela229、CaSki和Hela细胞）中EIF3D mRNA表达水平上调。EIF3D对体外宫颈癌模型细胞生长及Warburg效应有促进作用。EIF3D与GRP78相互作用降低体外宫颈癌模型GRP78活性。抑制GRP78可降低EIF3D对体外宫颈癌模型Warburg效应的影响。我们的研究发现EIF3D在体外宫颈癌模型中促进细胞生长和Warburg效应，抑制EIF3D代表了治疗宫颈癌的潜在治疗策略。关于这个主题我们已经知道了什么？宫颈癌的发病率在全球所有女性肿瘤中排名第三，在发展中国家排名第二。这项研究的结果补充了什么？本研究通过体内和体外模型研究了EIF3D对宫颈癌细胞进展的影响及其潜在机制。这些发现对临床实践和/或进一步研究的意义是什么？EIF3D在体外宫颈癌模型中促进细胞生长和Warburg效应，抑制EIF3D是治疗宫颈癌的一种潜在的治疗策略。
Abstract The incidence of cervical cancer ranks third among all female tumours globally and second in developing countries. However, the role of eukaryotic translation initiation factor 3 subunit D (EIF3D) in cervical carcinoma is unknown. This study investigated the effects of EIF3D on cell progression of cervical carcinoma and its underlying mechanism in vivo and vitro models. There were increases of EIF3D expression mRNA and protein expression levels in patients with cervical carcinoma. Disease-free survival (DFS) and overall surviva (OS) of EIF3D lower expression in patients with cervical carcinoma was higher than those of EIF3D higher expression. EIF3D mRNA expression levels in cervical carcinoma cell lines (AV3, Hela229, CaSki and Hela cells) were up-regulated, compared with cervical normal cell line (UVECs). EIF3D promoted cell growth and Warburg effect in vitro model of cervical carcinoma. EIF3D interacting with GRP78 to reduce the activity of GRP78 in vitro model of cervical carcinoma. The inhibition of GRP78 reduced the effects of EIF3D on Warburg effect in vitro model of cervical carcinoma. Our work identifies EIF3D promoted cell growth and Warburg effect in vitro model of cervical carcinoma and the inhibition of EIF3D represents a potential therapeutic strategy for the treatment of cervical carcinoma. IMPACT STATEMENT What is already known on this subject? The incidence of cervical cancer ranks third among all female tumours globally and second in developing countries. What do the results of this study add? This study investigated the effects of EIF3D on cell progression of cervical carcinoma and its underlying mechanism in vivo and vitro models. What are the implications of these findings for clinical practice and/or further research? EIF3D promoted cell growth and Warburg effect in vitro model of cervical carcinoma and the inhibition of EIF3D represents a potential therapeutic strategy for the treatment of cervical carcinoma.