A fast fiber enhancer exists in the muscle regulatory factor 4 gene promoter
A fast fiber enhancer exists in the muscle regulatory factor 4 gene promoter
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DOI：
10.1016/s0006-291x(02)02571-8
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发表时间：
2002-11-22
影响因子：
3.1
通讯作者：
Konieczny, SF
中科院分区：
文献类型：
--
作者：
Pin, CL;Konieczny, SF
骨骼肌的发育是一个高度调节的过程，由四种肌源性调节因子（MRF）MyoD、myf-5、肌细胞生成素和MRF 4控制。虽然这些因素表现出一些独特的功能，他们的个人活动的一部分，可以归因于不同的时间和空间的表达模式。为了描述控制MRFs表达的因素，我们已经开始对MRF 4基因启动子进行分子解剖。通过生成的启动子/报告基因构建体，我们表明，一个853 bp的片段，驻留在4 kb的MRF 4转录起始位点（853 AV）的上游，是能够增强基础MRF 4启动子的表达3-4倍，在肌细胞培养。对转基因小鼠中853 AV增强子的分析表明，该区域主要在快肌纤维中驱动MRF 4基因表达，这表明正常成人MRF 4表达模式受多种可能决定纤维类型特异性的控制元件调节。(C)2002 Elsevier Science（美国）。All rights reserved.
The development of skeletal muscle is a highly regulated process governed by the four myogenic regulatory factors (MRFs) MyoD, myf-5, myogenin, and MRF4. While these factors exhibit some unique functions, part of their individual activity can be attributed to different temporal and spatial expression patterns. To delineate the factors that control expression of the MRFs, we have begun a molecular dissection of the MRF4 gene promoter. Through the generation of promoter/reporter gene constructs, we show that an 853 bp fragment, residing 4 kb upstream of the MRF4 transcriptional start site (853AV), is able to enhance expression of the basal MRF4 promoter 3-4-fold in myogenic cell cultures. Analysis of the 853AV enhancer in transgenic mice indicates that this region drives MRF4 gene expression primarily in fast muscle fibers, suggesting that the normal adult MRF4 expression pattern is regulated by a variety of control elements that may dictate fiber-type specificity. (C) 2002 Elsevier Science (USA). All rights reserved.