Fully integrated glass microfluidic device for performing high-efficiency capillary electrophoresis and electrospray ionization mass spectrometry.
Fully integrated glass microfluidic device for performing high-efficiency capillary electrophoresis and electrospray ionization mass spectrometry.
复制标题
DOI：
10.1021/ac800428w
复制
发表时间：
2008-09-15
影响因子：
7.4
通讯作者：
Ramsey, J. M.
中科院分区：
文献类型：
--
作者：
Mellors, J. S.;Gorbounov, V.;Ramsey, R. S.;Ramsey, J. M.
已经开发出一种微加工装置，其中电喷雾电离直接从矩形玻璃微芯片的角进行。该设备允许高效电动驱动分离直接耦合到质谱仪（MS），而无需使用外部压力源或插入毛细管喷雾头。基于电动的液压泵集成在芯片上，其将洗脱材料引导到单片集成的喷嘴尖端。带正电荷的表面涂层PolyE-323用于防止与肽和蛋白质的表面相互作用，并逆转分离通道中的电渗流。该装置已被用来进行微芯片CE-MS分析的肽和蛋白质的效率超过200 000理论板（1 000 000板/米）。的灵敏度和稳定性的微型ESI源被认为是商业拉熔融石英毛细管纳米喷雾源。
A microfabricated device has been developed in which electrospray ionization is performed directly from the corner of a rectangular glass microchip. The device allows highly efficient electrokinetically driven separations to be coupled directly to a mass spectrometer (MS) without the use of external pressure sources or the insertion of capillary spray tips. An electrokinetic-based hydraulic pump is integrated on the chip that directs eluting materials to the monolithically integrated spray tip. A positively charged surface coating, PolyE-323, is used to prevent surface interactions with peptides and proteins and to reverse the electroosmotic flow in the separation channel. The device has been used to perform microchip CE-MS analysis of peptides and proteins with efficiencies over 200 000 theoretical plates (1 000 000 plates/m). The sensitivity and stability of the microfabricated ESI source were found to be comparable to that of commercial pulled fused-silica capillary nanospray sources.
登录
查看更多内容
影响因子：
7.4
作者：
Bings, NH;Wang, C;Harrison, DJ
通讯作者：
Harrison, DJ
影响因子：
7.4
作者：
Deng, YZ;Henion, J;Harrison, DJ
通讯作者：
Harrison, DJ
影响因子：
7.4
作者：
Griffiths, SK;Nilson, RH
通讯作者：
Nilson, RH
影响因子：
7.4
作者：
Schultz, GA;Corso, TN;Zhang, S
通讯作者：
Zhang, S
影响因子：
7.4
作者：
Li, JJ;Kelly, JF;Thibault, P
通讯作者：
Thibault, P