Seroprevalence of avian influenza virus, infectious bronchitis virus, reovirus, avian pneumovirus, infectious laryngotracheitis virus, and avian leukosis virus in Nigerian poultry
Seroprevalence of avian influenza virus, infectious bronchitis virus, reovirus, avian pneumovirus, infectious laryngotracheitis virus, and avian leukosis virus in Nigerian poultry
复制标题
DOI：
10.1637/7412-071505r.1
复制
发表时间：
2006-06-01
期刊：
影响因子：
1.4
通讯作者：
Muller, CP
中科院分区：
文献类型：
--
作者：
Owoade, AA;Ducatez, MF;Muller, CP
对尼日利亚8个养鸡场的9个种鸡场、14个肉鸡场、28个小母鸡场、11个蛋鸡场和3个公鸡场的鸡群进行了传染性支气管炎病毒（IBV）、禽呼肠孤病毒（Avian Reovirus）、禽肺炎病毒（APV）、传染性喉气管炎病毒（ILTV）、禽流感病毒（AIV）和禽白血病病毒（ALV）的抗体血清阳性率检测。1999年至2004年期间收集了血清样本，并使用商业酶联免疫吸附测定（ELISA）试剂盒检测抗体。IBV的血清阳性率非常高（84%）;呼肠孤病毒（41%）、APV（40%）和ILTV（20%）居中; ALV抗体非常低（< 5%）。用商业ELISA法检测，部分鸡群的AIV抗体阳性率高达63%。然而，更具体的检测没有确认AIV抗体，表明所有检测的鸡群都没有禽流感抗体。鸡似乎首先被IBV感染（约7周龄），然后在12周龄时被呼肠孤病毒感染，然后被APV（25周）和ILTV（30周）感染。这是西非首次报告上述病毒的血清学证据。有必要开展进一步研究，以评估这些禽流感病毒造成的经济损失以及采取对策的成本和效益。
Eight poultry farms in Nigeria, including chickens from nine breeder, 14 broiler, 28 pullet, I I layer, and three cockerel flocks, were tested for antibody seroprevalence to the following poultry viruses of potential economic importance: infectious bronchitis virus (IBV), avian reovirus, avian pneumovirus (APV), infectious laryngotracheitis virus (ILTV), avian influenza virus (AIV), and avian leukosis virus (ALV). Serum samples were collected between 1999 and 2004 and were tested for antibodies using commercial enzyme-linked immunosorbent assay (ELISA) kits. Seroprevalence was very high for IBV (84%); intermediate for reovirus (41%), APV (40%), and ILTV (20%); and very low for ALV (< 5%) antibodies. By commercial ELISA, the seroprevalence of antibodies against AIV was, in some flocks, up to 63%. However, more specific assays did not confirm AIV antibodies, indicating that all flocks tested were free of avian influenza antibodies. Birds seemed to be first infected by IBV (at about 7 wk of age), then by reovirus at 12 wk, before they became infected by APV (week 25) and ILTV (week 30). This is the first report of serological evidence of the above viruses in West Africa. Further studies are necessary to assess economic losses due to these avian viruses and the costs and benefits of countermeasures.