Definition of the attl1 site of class 1 integrons
Definition of the attl1 site of class 1 integrons
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DOI：
10.1099/00221287-146-11-2855
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发表时间：
2000-11-01
期刊：
影响因子：
--
通讯作者：
Hall, RM
中科院分区：
文献类型：
--
作者：
Partridge, SR;Recchia, GD;Hall, RM
整合子编码的整合酶识别两种不同类型的重组位点：在整合子中发现的attl位点和在整合子相关基因盒中发现的59-碱基元件（59-be）家族的成员。1类整合子整合酶Intl 1催化attl 1和一个59-be、两个59-be或两个attl 1位点之间的重组，但涉及两个attl 1位点的事件的效率低于59-be参与的反应。完整的attl 1位点是与59-be位点高效重组所必需的。它的长度为65 bp，包括一个简单的网站，由一对反向Intl 1结合结构域，以及两个进一步的直接定向Intl 1结合位点指定的强和弱。然而，仅包含简单位点的较小区域足以支持与完整attl 1伴侣的较低水平的重组，并且决定attl 1方向的特征位于该区域内。attl 1位点和59-be之间的不寻常反应似乎是导致59-be中心区域丢失的原因，从而产生两个相邻基因盒的潜在融合。
Integron-encoded integrases recognize two distinct types of recombination site: attl sites, found in integrons, and members of the 59-base element (59-be) family, found in the integron-associated gene cassettes. The class 1 integron integrase, Intl1, catalyses recombination between attl1 and a 59-be, two 59-be, or two attl1 sites, but events involving two attl1 sites are less efficient than the reactions in which a 59-be participates. The full attl1 site is required for high-efficiency recombination with a 59-be site. It is 65 bp in length and includes a simple site, consisting of a pair of inversely oriented Intl1-binding domains, together with two further directly oriented Intl1-binding sites designated strong and weak. However, a smaller region that contains only the simple site is sufficient to support a lower level of recombination with a complete attl1 partner and the features that determine the orientation of attl1 reside within this region. An unusual reaction between the attl1 site and a 59-be appears to be responsible for the loss of the central region of a 59-be to create a potential fusion of two adjacent gene cassettes.