Suppression of Specific IgE Antibody Responses by Liposome-Conjugated Ovalbumin in Mice Sensitized with Ovalbumin via the Respiratory Tract
Suppression of Specific IgE Antibody Responses by Liposome-Conjugated Ovalbumin in Mice Sensitized with Ovalbumin via the Respiratory Tract
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脂质体缀合的卵清蛋白对通过呼吸道卵清蛋白致敏的小鼠的特异性 IgE 抗体反应的抑制
DOI：
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发表时间：
2000
影响因子：
2.8
通讯作者：
S. Tamura
中科院分区：
文献类型：
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作者：
T. Yoshikawa;T. Uchida;S. Naito;A. Horino;M. Taneichi;H. Kato;K. Komuro;Y. Nakano;M. Mori;S. Nishinohara;J. Chiba;T. Kurata;S. Tamura
背景资料：以前，我们已经表明，卵清蛋白（OVA）与霍乱毒素（CT）一起鼻内给药废除鼻腔耐受OVA，导致在诱导特异性IgE抗体（Ab）的反应，和腹腔注射的OVA与脂质体（OVA-脂质体）耦合诱导的选择性抑制IgE抗体对OVA的反应。OVA-脂质体是否抑制通过呼吸道用CT结合的OVA致敏的小鼠中的抗OVA IgE Ab应答仍有待澄清。研究方法：在一些实验中，以2周的间隔（第0、2和4周）鼻内给予小鼠三次OVA、脂质体或OVA-脂质体（有或没有CT）。在其他实验中，在CT组合的OVA（第0周）之前2天或之后1周和3周鼻内给予小鼠OVA-脂质体，其以2周的间隔鼻内给予三次（第0、2和4周）。在第三次施用CT组合的OVA（第0周）后两周，测定鼻洗液和血清伊加、IgG和IgE Ab应答。结果如下：用OVA-脂质体预处理抑制IgE Ab对CT结合的OVA的反应，具有显着高的鼻伊加和血清IgG Ab的产生。此外，在CT组合的OVA施用后1周和3周用OVA-脂质体治疗也抑制IgE Ab应答。OVA脂质体抑制抗OVA IgE Ab的产生，同时增强特异性伊加和IgG（IgG 1，尤其是IgG 2a）Ab的产生。结论：免疫后处理与OVA脂质体，以及免疫前处理，抑制特异性IgE抗体反应，在小鼠鼻内致敏与CT结合的OVA。与脂质体结合的过敏原可能适用于预防人类对吸入或饮食抗原过敏的发展。
Background: Previously we have shown that intranasal administration of ovalbumin (OVA) together with cholera toxin (CT) abrogates nasal tolerance to OVA, resulting in the induction of specific IgE antibody (Ab) responses, and that intraperitoneal injection of OVA coupled with liposomes (OVA-liposomes) induces a selective suppression of IgE Ab responses to OVA. Whether OVA-liposomes suppress anti-OVA IgE Ab responses in mice sensitized with CT-combined OVA via the respiratory tract remains to be clarified. Methods: In some experiments, mice were given OVA, liposomes or OVA-liposomes with or without CT intranasally three times, at 2-week intervals (weeks 0, 2 and 4). In other experiments, mice were given OVA-liposomes intranasally 2 days before or 1 and 3 weeks after CT-combined OVA (week 0), which was administered intranasally three times, at 2-week intervals (weeks 0, 2 and 4). Two weeks after the third administration of CT-combined OVA (week 0), nasal wash and serum IgA, IgG and IgE Ab responses were assayed. Results: Pretreatment with OVA-liposomes suppressed IgE Ab responses to CT-combined OVA, with a significantly high production of both nasal IgA and serum IgG Abs. Moreover, treatment with OVA-liposomes 1 and 3 weeks after CT-combined OVA administration also suppressed IgE Ab responses. The suppression of anti-OVA IgE Ab production by OVA-liposomes was accompanied by a simultaneous enhancement of specific IgA and IgG (IgG1, and especially IgG2a) Ab production. Conclusions: Postimmunization treatment with OVA-liposomes, as well as preimmunization treatment, suppressed specific IgE Ab responses in mice sensitized intranasally with CT-combined OVA. Allergens conjugated to liposomes may be appropriate for preventing the development of allergies to inhaled or dietary antigens in humans.
霍乱毒素喂养不会诱导小鼠的口服耐受性，并且消除了对不相关蛋白抗原的口服耐受性。
DOI：
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发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Elson,CO;Ealding,W
通讯作者：
Ealding,W
影响因子：
4.4
作者：
Yingzi Cong;C. Weaver;C. Elson;C. Elson
通讯作者：
Yingzi Cong;C. Weaver;C. Elson;C. Elson
当与蛋白质免疫原共同施用时，IL-1 是粘膜和全身免疫反应的有效佐剂。
DOI：
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发表时间：
1999
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Staats,HF;EnnisJr,FA
通讯作者：
EnnisJr,FA