Selective 2-[18F]fluorodopa uptake for melanogenesis in murine metastatic melanomas.
Selective 2-[18F]fluorodopa uptake for melanogenesis in murine metastatic melanomas.
复制标题
选择性 2-[18F]氟多巴摄取用于小鼠转移性黑色素瘤的黑色素生成。
作者：
K. Ishiwata;K. Kubota;R. Kubota;R. Iwata;T. Takahashi;T. Ido
用携带两种B16黑色素瘤的小鼠研究3,4-二羟基-2-[18F]氟- l -苯丙氨酸（2-[18F]FDOPA）摄取与黑色素形成之间的关系：B16- f1具有更高的黑色素合成能力，比更高转移性的B16- f10生长速度更慢。注射后1小时，B16-F1对2-[18F]FDOPA的摄取明显高于B16-F10，对[14C] 2-脱氧-2-氟-d -葡萄糖和[3H]胸腺嘧啶的摄取呈反比关系。2-[18F]FDOPA摄取和酸不溶性放射性的F1-to-F10比值在6小时增加到约5，与黑色素含量平行。FM3A乳腺癌表现出与B16-F10相似的2-[18F]FDOPA摄取，但没有酸不溶性放射性。在D，L-DOPA加载下，注射后1小时FM3A摄取减少55%，显著大于两种黑色素瘤中20%的减少。o -甲基化2-[18F]FDOPA是所有肿瘤中主要的酸溶性代谢物。全身放射自显像对两种黑色素瘤鉴别清楚。2-[18F]FDOPA可能是一种很有前途的黑素形成选择性成像示踪剂。
The relationship between 3,4-dihydroxy-2-[18F]fluoro-L-phenylalanine (2-[18F]FDOPA) uptake and melanogenesis was studied using mice bearing two B16 melanomas: B16-F1 has a higher melanin synthesis ability and a slower growing rate than the higher metastatic B16-F10. A significantly higher 2-[18F]FDOPA uptake by B16-F1 than by B16-F10 and a reverse relationship for the uptake of [14C] 2-deoxy-2-fluoro-D-glucose and [3H]thymidine were observed 1 hr postinjection. F1-to-F10 ratios of both the 2-[18F]FDOPA uptake and the acid-insoluble radioactivity increased to about 5 at 6 hr, which paralleled the melanin content. FM3A mammary carcinoma showed a 2-[18F]FDOPA uptake similar to the B16-F10 but without the acid-insoluble radioactivity. With D,L-DOPA loading, a 55% decreased uptake by FM3A 1 hr postinjection was significantly greater than the 20% reduction in both melanomas. O-Methylated 2-[18F]FDOPA was a predominant acid-soluble metabolite in all tumors. Whole-body autoradiography discriminated the two melanomas clearly. 2-[18F]FDOPA may be a promising tracer for the selective imaging of melanogenesis.