Increased replication of respiratory syncytial virus in the presence of cytokeratin 8 and 18.
Increased replication of respiratory syncytial virus in the presence of cytokeratin 8 and 18.
复制标题
在细胞角蛋白 8 和 18 存在的情况下，呼吸道合胞病毒的复制增加。
DOI：
10.1002/jmv.23196
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Matsuyama S.
中科院分区：
文献类型：
--
作者：
Shirato K;Ujike M;Kawase M;Matsuyama S.
此前有报道称，呼吸道合胞病毒（RSV）的生产性病毒感染、病毒蛋白合成和病毒RNA复制在两种人上皮细胞系（HEp-2和A549）中有效，但在人肥大细胞系HMC-1中无效。基于这些观察结果，假设HMC-1细胞缺乏RSV复制所需的机制。为了鉴定RSV复制所需的宿主因子，使用A549、HEp-2和HMC-1细胞进行cDNA扣除，并将细胞角蛋白18（C18）鉴定为候选宿主因子。由于C18通常在具有细胞角蛋白8（C8）的单纯上皮中表达，因此建立了组成型表达C18和C8的HMC-1细胞（HMC-1-C8/18），以评价C8/18在RSV复制中的作用。在HMC-1-C8/18细胞中，RSV RNA复制增加，并且在RSV spinoculation后细胞组分中产生的感染性病毒的量也增加，而在C18表达被敲低的A549细胞中，RSV产生减少。这些数据表明，RSV的复制在C8/18的存在下增加。医学病毒学杂志84：365-370，2012.© 2011 Wiley Periodicals，Inc.
Previously, it was reported that productive viral infection, viral protein synthesis, and viral RNA replication of respiratory syncytial virus (RSV) operated efficiently in two human epithelial cell lines (HEp‐2 and A549), but not in a human mast‐cell line, HMC‐1. Based on these observations, it was hypothesized that HMC‐1 cells lack the machinery required for RSV replication. To identify the host factors required for RSV replication, cDNA subtraction using A549, HEp‐2, and HMC‐1 cells was performed, and cytokeratin 18 (C18) was identified as a candidate host factor. Because C18 is generally expressed in simple epithelia with cytokeratin 8 (C8), HMC‐1 cells that constitutively express C18 and C8 (HMC‐1‐C8/18) were established to evaluate the role of C8/18 in RSV replication. In HMC‐1‐C8/18 cells, RSV RNA replication was increased, and the amount of infective virus produced was also increased in the cellular fraction after RSV spinoculation, whereas RSV production was decreased in A549 cells in which C18 expression was knocked down. These data suggest that the replication of RSV increases in the presence of C8/18. J. Med. Virol. 84:365–370, 2012. © 2011 Wiley Periodicals, Inc.