Assay of Messenger Activity of RNA from Bacillus subtilis in Heterologous Cell-free System and Its Relation to α-Amylase Productivity
Assay of Messenger Activity of RNA from Bacillus subtilis in Heterologous Cell-free System and Its Relation to α-Amylase Productivity
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异源无细胞体系中枯草芽孢杆菌RNA信使活性测定及其与α-淀粉酶生产率的关系
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
B. Maruo
中科院分区：
文献类型：
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作者：
Y. Nagata;H. Yamauchi;B. Maruo
枯草芽孢杆菌菌株M15、TM 23和T2 N26分别以0、50和350单位/小时/ml的速率产生α-淀粉酶。从这些菌株的细胞中提取的RNA用作信使来源。这些来源刺激掺入标记的氨基酸在异源的无细胞蛋白质合成系统从大肠杆菌制备。用抗α-淀粉酶血清沉淀放射性标记的蛋白质，在体内和体外测定α-淀粉酶特异性mRNA的量。结果表明，B.枯草杆菌在很大程度上反映了α-淀粉酶mRNA的含量。
Bacillus subtilis strains M15, TM23 and T2N26 produced α-amylase at rates of 0, 50 and 350 units/hr/ml, respectively. RNA extracted from cells of these strains were used as messenger sources. These sources stimulated the incorporation of labeled amino acids in a heterologous cell-free protein synthesizing system prepared from Escherichia coli. The amounts of mRNA specific for α-amylase were estimated in vivo and in vitro by precipitation of radio-labeled proteins with anti-α-amylase serum. The results suggest that the capability of α-amylase synthesis in B. subtilis largely reflected the content of mRNA for α-amylase.