[Molecular genetic marker identification of traditional Chinese drug hippocampus].
[Molecular genetic marker identification of traditional Chinese drug hippocampus].
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
P. Wu;K. Zhou;Z. Zhang;L. Xu
中科院分区：
文献类型：
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作者：
P. Wu;K. Zhou;Z. Zhang;L. Xu
采用古DNA技术从5种中药海马中提取DNA。使用聚合酶链反应（PCR）方法从DNA提取物中扩增12S rRNA基因片段和细胞色素b基因片段。进行限制性片段长度多态性(RFLP)分析和DNA序列分析。 RFLP分析方法可以鉴定2种海马。通过DNA测序方法产生的分子遗传标记可以识别所有5种海马。该方法对于其他兽药的鉴定具有一定的参考价值。
Ancient DNA technique was used to extract DNA from 5 species of Chinese traditional drugs Hippocampus. The 12S rRNA gene fragment and cytochrome b gene fragment were amplified from DNA extract using Polymerase Chain Reaction (PCR) method. Restriction Fragment Length Polymorphism (RFLP) analysis and DNA sequence analysis were performed. The RFLP analysis method can identify 2 species of Hippocampus. The molecular genetic markers produced by DNA sequence method can identify all 5 species of Hippocampus. This method will be valuable for the identification of other animal drugs.