Target of Rapamycin Signaling Regulates Metabolism, Growth, and Life Span in Arabidopsis
Target of Rapamycin Signaling Regulates Metabolism, Growth, and Life Span in Arabidopsis
复制标题
DOI：
10.1105/tpc.112.107144
复制
发表时间：
2012-12-01
期刊：
影响因子：
11.6
通讯作者：
Datla, Raju
中科院分区：
文献类型：
--
作者：
Ren, Maozhi;Venglat, Prakash;Datla, Raju
雷帕霉素靶蛋白（TOR）是一种重要的营养和能量传感器，调节酵母和动物的生长和寿命。在植物中，生长和寿命不仅与从土壤中获取营养和通过光合作用产生营养交织在一起，而且与其独特的发育和分化模式交织在一起。职权范围如何在这些进程中发挥作用尚未确定。为了获得进一步的了解，开发了表达酵母FK 506结合蛋白12的雷帕霉素敏感的转基因拟南芥系（BP 12）。通过雷帕霉素抑制BP 12植物中的TOR导致较慢的整体根、叶和芽生长和发育，导致较差的营养吸收和光能利用。在野生型拟南芥中营养物质可用性和光能供应的实验限制产生了用TOR敲低植物观察到的表型，表明TOR信号传导和营养/光能状态之间的联系。基因和生理学研究以及TOR抑制株系的RNA测序和代谢产物分析表明，TOR通过重组细胞生长、碳氮代谢、基因表达以及rRNA和蛋白质合成来调节拟南芥的发育和寿命。核糖体蛋白S6（RPS 6）突变体的功能获得和丧失还表明，TOR功能涉及RPS 6介导的营养和光依赖性生长和寿命在拟南芥。
Target of Rapamycin (TOR) is a major nutrition and energy sensor that regulates growth and life span in yeast and animals. In plants, growth and life span are intertwined not only with nutrient acquisition from the soil and nutrition generation via photosynthesis but also with their unique modes of development and differentiation. How TOR functions in these processes has not yet been determined. To gain further insights, rapamycin-sensitive transgenic Arabidopsis thaliana lines (BP12) expressing yeast FK506 Binding Protein12 were developed. Inhibition of TOR in BP12 plants by rapamycin resulted in slower overall root, leaf, and shoot growth and development leading to poor nutrient uptake and light energy utilization. Experimental limitation of nutrient availability and light energy supply in wild-type Arabidopsis produced phenotypes observed with TOR knockdown plants, indicating a link between TOR signaling and nutrition/light energy status. Genetic and physiological studies together with RNA sequencing and metabolite analysis of TOR-suppressed lines revealed that TOR regulates development and life span in Arabidopsis by restructuring cell growth, carbon and nitrogen metabolism, gene expression, and rRNA and protein synthesis. Gain-and loss-of-function Ribosomal Protein S6 (RPS6) mutants additionally show that TOR function involves RPS6-mediated nutrition and light-dependent growth and life span in Arabidopsis.