Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues.
Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues.
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四倍体胚胎干细胞可以促进嵌合胎儿和嵌合胚胎外组织的发育。
DOI：
10.1038/s41598-017-02783-0
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发表时间：
2017-06-08
影响因子：
4.6
通讯作者：
Han J
中科院分区：
文献类型：
--
作者：
Wen B;Li R;Cheng K;Li E;Zhang S;Xiang J;Wang Y;Han J
我们的研究检测了通过将表达绿色荧光蛋白(GFP)的四倍体胚胎干细胞(ESCs)注射到二倍体胚胎中而产生的嵌合体的体内嵌合和存活能力。在结膜后3.5d和4.5dPC时，四倍体胚胎干细胞与绿色荧光蛋白标记的二倍体胚胎干细胞一样能够形成内细胞团。在6.5dpc、8.0dpc和10.5dpc 时，四倍体胚胎干细胞与二倍体胚胎干细胞出现在同一位置。四倍体ESC嵌合体胚胎外组织和胚胎中的GFP细胞经荧光激活细胞分选(FACS)鉴定为四倍体。此外，在13.5dpc和16.5dpc时，四倍体胚胎干细胞促进了胎盘、胚膜和脐带的发育，而四倍体胚胎干细胞嵌合体胎儿中只有很少的绿色荧光蛋白细胞。我们进一步发现，四倍体胚胎干细胞的增殖速度慢于二倍体胚胎干细胞。此外，与二倍体胚胎干细胞相比，四倍体胚胎干细胞在三个胚层和滋养层中的相对mRNA表达均异常。总之，四倍体胚胎干细胞增殖缓慢和分化潜能异常可能是其存活率和嵌合能力差的两个原因。
Our study examined the in vivo chimeric and survival capacities of chimeras created by injecting tetraploid embryonic stem cells (ESCs) expressing green fluorescent protein (GFP) into diploid embryos. At 3.5 days post-coitum (dpc) and 4.5 dpc, the tetraploid ESCs were able to contribute to the inner cell mass (ICM) just as diploid ESCs tagged with GFP. At 6.5 dpc, 8.0 dpc and 10.5 dpc, the tetraploid ESCs manifested in the same location as the diploid ESCs. The GFP cells in the extraembryonic tissues and fetuses of tetraploid ESC chimeras were tetraploid as determined by fluorescence activated cell sorting (FACS). Furthermore, tetraploid ESCs contributed to the development of the placenta, embryolemma and umbilical cord at 13.5 dpc and 16.5 dpc; however, very less GFP cells were found in the fetuses of tetraploid ESC chimeras. We further found that the proliferation of tetraploid ESCs was slower than that of diploid ESCs. In addition, the relative mRNA expression in the three germ layers and the trophoblast was abnormal in the EBs of tetraploid ESCs compared with diploid ESCs. In short, slower proliferation and abnormal differentiation potential of tetraploid ESCs might be two of the reasons for their poor survival and chimeric capacities.