Exact analysis of competition ligand binding by displacement isothermal titration calorimetry
Exact analysis of competition ligand binding by displacement isothermal titration calorimetry
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DOI：
10.1006/abio.1999.4402
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发表时间：
2000-01-15
影响因子：
2.9
通讯作者：
Sigurskjold, BW
中科院分区：
文献类型：
--
作者：
Sigurskjold, BW
提出了位移等温滴定量热数据的最小二乘非线性回归分析的一种严密方法。该方法可以将被另一个结合配体竞争抑制的配体与具有n个相同和独立结合位点的分子的结合等温线拟合。该方法没有其他假设，也没有近似值。作为一个例子，分析了以前发表的阿卡波糖与糖淀粉酶的强结合数据。回归方程已经为MicroCal，Inc.的广泛使用的滴定量热计提供的Origin软件编程，作者可以通过电子邮件请求免费获得带有说明的Origin函数定义文件。(C)2000年学术出版社。
A rigorous method for the least-squares nonlinear regression analysis of displacement isothermal titration calorimetric data is presented. The method can fit the binding isotherm of a ligand which is competitively inhibited in its binding by another bound ligand to a molecule with n identical and independent binding sites. There are no other assumptions for the method and no approximations. Analysis of previously published data of the strong binding of acarbose to glucoamylase is presented as an example. The regression equations have been programmed for the Origin software supplied with the widely used titration calorimeters from Microcal, Inc., and an Origin Function Definition File with instructions is freely available from the author upon e-mail request. (C) 2000 Academic Press.